硫酸卡那霉素和氟苯尼考联合用药的急性毒效应评价  

Evaluation of the Acute Toxic Effects of Combined Medication of Kanamycin Sulfate with Florfenicol

作　　者：杨熠[1] 高超[1] 任丽娟[1] 帕力达.亚森 马晓洋[1] 米克热木.沙衣布扎提 

机构地区：[1]新疆农业大学,新疆乌鲁木齐830052

出　　处：《畜牧与饲料科学》2016年第3期7-10,共4页Animal Husbandry and Feed Science

基　　金：国家自然科学基金资助项目(31460677)

摘　　要：通过对小鼠进行硫酸卡那霉素和氟苯尼考联合应用的急性毒效应评价,初步探讨这2种抗菌药联合应用的合理性,以期为进一步评价其安全指标提供可靠依据。经查证,硫酸卡那霉素对小鼠腹腔注射的LD_（50）为1 973.95 mg/kg,其95%可信限为1 969.22~1 978.71 mg/kg;氟苯尼考对小鼠腹腔注射的LD_（50）为636.68 mg/kg,其95%可信限为538.5~735.1 mg/kg,该试验利用等效应线图法求出硫酸卡那霉素和氟苯尼考联合用药时的LD_（50）。试验表明,硫酸卡那霉素和氟苯尼考联合用药中,硫酸卡那霉素对小鼠腹腔注射的LD_（50）为510.51mg/kg,其95%可信限为437.52~595.66 mg/kg;氟苯尼考对小鼠腹腔注射的LD_（50）为765.60 mg/kg,其95%可信限为656.15~893.31 mg/kg。结果显示,硫酸卡那霉素和氟苯尼考联合应用后的LD_（50）剂量直线交点落在95%可信限上限之外,即这2种抗菌药的联合用药产生毒效应颉颃,初步认为联合使用具有一定的合理性与可靠性。In order to evaluate the clinical rationality of combined medication of kanamycin sulfate with florfenicol and to provide solid basis for further assessment of its safety indicators, the acute toxic effects of the combined medication of these two drugs on mice was investigated. The reported LD_（50） of kanamycin sulfate and florfenicol by intraperitoneal injection were 1 973.95 mg/kg and 636.68 mg/kg respectively, and the reported 95% confidence limit of kanamycin sulfate and florfenicol by intraperitoneal injection were 1 969.22-1 978.71 mg/kg and 538.5-735.1 mg/kg respectively. Based on these reported pharmacological parameters, the LD_（50） of combined medication of kanamycin sulfate with florfenicol was determined by equivalent diagram method.The results showed that in the combined medication, the LD_（50） of kanamycin sulfate by intraperitoneal injection in mice was 510.51mg/kg and the 95% confidence limit ranged from 437.52 to 595.66 mg/kg; the LD_（50） of florfenicol by intraperitoneal injection in mice was 765.60 mg/kg and the 95% confidence limit ranged from 656.15 to 893.31 mg/kg. Furthermore, the intersection of LD_（50） dose line of combined medication of kanamycin sulfate and florfenicol fell outside the upper limit of 95% confidence limit,indicating that the combined medication of these two antibiotics produced antagonism of toxic effect. These results suggest the clinical rationality and reliability of combined medication of kanamycin sulfate with florfenicol.

关 键 词：半数致死量 联合用药 硫酸卡那霉素:氟苯尼考 

分 类 号：R969.2[医药卫生—药理学]