PCR-线性探针杂交酶显色法在结核病诊断与耐药性检测中的应用研究  

Application of PCR-line probe hybridization enzyme coloration assay in the diagnosis of tuberculosis and the detection of drug resistance

作　　者：郭明日[1,2] 王冰冰[1] 张丽霞[1] 张立[1] 周洪经[1] 

机构地区：[1]天津市海河医院检验科天津医科大学海河临床学院,天津300350 [2]天津市呼吸疾病研究所,天津300350

出　　处：《中华医院感染学杂志》2016年第7期1483-1485,共3页Chinese Journal of Nosocomiology

基　　金：天津市卫生局科技基金项目资助(2014KZ035)

摘　　要：目的对比PCR-线性探针杂交酶显色法(简称线性探针法)与BACTEC MGIT960液体培养及药敏在结核分枝杆菌检出率的差异,评价两种方法检测结核分枝杆菌对利福平和异烟肼耐药性的应用效果。方法选取医院结核科2013年8月-2015年5月369例确诊及疑似结核病的住院患者送检标本,同时采用线性探针法和BACTEC MGIT960对其进行结核分枝杆菌及利福平和异烟肼耐药性检测,数据采用SPSS 13.0软件进行统计分析。结果线性探针法检测结核分枝杆菌对利福平耐药性的灵敏度为90.4%、特异度为97.3%、Kappa值为0.872;对异烟肼耐药性检测的灵敏度为86.6%、特异度为96.3%、Kappa值为0.841,两种方法在利福平和异烟肼耐药性检测中具有较好的一致性。结论 PCR-线性探针杂交酶显色法是一种快速、准确的结核分枝杆菌及其对利福平和异烟肼耐药性的诊断方法,对耐多药结核病的快速诊断具有显著的优点。OBJECTIVE To evaluate the value of PCR-line probe hybridization enzyme coloration assay（hereafter referred to as line probe assay,LPA）,BACTEC MGIT 960 liquid culture and drug sensitivity test in Mycobacterium tuberculosis detection and of its resistance to rifampicin（RIF）and isoniazid（INH）.METHODS A total of 369 cases of confirmed and suspected TB inpatients of Tuberculosis Department in our hospital were chosen from Aug.2013 to May 2015 and detected by line probe assay（LPA）and BACTEC MGIT 960 at the same time.SPSS 13.0was adopted for statistical analysis.RESULTS Totally 214 cases and 216 cases of specimens were positive of M.tuberculosis detected by LPA and BACTEC MGIT960,and the positive rates were 57.9% and 58.5%respectively.There was no significant difference between the two methods.Using results of M.tuberculosis drug susceptibility from BACTEC MGIT 960 as reference,the sensitivity and specificity of LPA to RIF were 90.4% and97.3% respectively,kappa value was 0.872;the sensitivity and specificity of LPA to INH were 86.6% and96.3% respectively,kappa value was 0.841,therefore,the consistency of the two methods in resistance detection of RIF and INH were excellent.CONCLUSIONPCR-line probe hybridization enzyme coloration assay（LPA）was a fast and accurate molecular method to identify M.tuberculosis and their resistance to RIF and INH,therefore is effective in the rapid diagnosis of multi-drug resistance M.tuberculosis（MDR-TB）.

关 键 词：线性探针杂交酶显色法 结核分枝杆菌 耐药性 

分 类 号：R378.91[医药卫生—病原生物学]