维生素C及其转运体SVCT在子痫前期发病机制中的作用研究  

The Research of Vitamin C and SVCT in the Pathogenesis of Preeclampsia

作　　者：陈娟娟[1] 程澄[1] 谢玉珍[1] 何青[1] 陈海霞[1] 李映桃[1] 

机构地区：[1]广州医科大学附属第三医院广东省产科重大疾病重点实验室,广东广州510150

出　　处：《实用妇产科杂志》2016年第4期282-285,共4页Journal of Practical Obstetrics and Gynecology

基　　金：广东省自然科学基金(编号:S2013010014810)

摘　　要：目的:通过前瞻性随机对照临床研究,探讨围生期维生素C的摄入及代谢与子痫前期(PE)的关系,探索PE的可能发病机制。方法:随机选取2011年9月至2014年3月广州医科大学附属第三医院住院孕妇344例,包括研究组(PE患者)170例、对照组(正常妊娠)174例。记录每日维生素C摄入量;收集其清晨中段尿、外周血、脐血及胎儿附属物(胎盘、胎膜、脐带),采用ELISA方法检测母体尿液、外周血及脐血血清维生素C含量,免疫组化法观察胎盘、胎膜、脐带依赖钠离子维生素C转运蛋白(SVCT)1及SVCT2的表达情况,PCR-RFLP法检测SLC23a1的多态性。结果:1研究组每日维生素C摄入量及尿液维生素C含量与对照组相比,差异无统计学意义(P>0.05);2研究组母体外周血维生素C含量明显高于对照组,差异有统计学意义(P<0.05);3研究组脐血维生素C含量低于对照组,差异有统计学意义(P<0.05);4研究组胎儿附属物SVCT1及SVCT2表达水平均较对照组低,差异有统计学意义(P<0.05);5研究组与对照组SLC23a1多态性在rs35817838、rs33972313、rs6886922和rs34521685位点上并无差异(P>0.05)。结论:胎儿附属物SVCT1及SVCT2表达水平降低,从而影响母体与胎儿之间的维生素C的转运,使得维生素C等抗氧化物不能有效到达胎盘,这可能是PE发病机制之一。Objective：To explore the relationship of vitamin C and preeclampsia and to research the pathogen- esis of preeclampsia by prospective randomized controlled clinical studies. Methods：344 pregnant women atten- ding at the Third Affiliated Hospital of Guangzhou Medical College hospital from 1st September 2011 to 1st March 2014,including research group（170 women with preeclampsia） and control group（174 norm pregnant women） were randomly selected. The daily intake of Vitamin C was recorded,and the urine,peripheral blood,cord blood, and fetal attachment（placenta, fetal membrane, and cord） collected in all the patients. The level of vitamin C in peripheral blood and cord blood were detected by ELISA,the expression of SVCT1 and SVCT2 on placenta, membrane and cord were detected by immunohistochemical, and the SNP of SLC23al were detected by PCR-RFLP. Results：①There were no significant difference on the vitamin C daily intake and the level of vitamin C in urine between the research group and the control group （ P 〉 0, 05）. ②The level of vitamin C in peripheral blood of research groupwas higher than that of the control group in pregnant of 34 weeks（ P〈0. 05）. ③The level of vitamin C in cord blood of research group was lower than that of the control group（P〈0.05）. ④The expres- sion of SVCT1 and SVCT2 in the fetal attachment of research group were lower than control group（ P 〈0.05）. ⑤There was no difference on the SLC23a1 SNP in rs35817838, rs33972313,rs6886922 and rs34521685 between the two groups（ P 〉 0. 05）. Conclusions： Preeclampsia is placenta-origin disease, the probably pathogenesis is that the decreasing of expression of SVCT1 and SVCT2 affects the transportation of the vitamin C between moth-er and the fetus,and subsequently decreases the vitamin C effectively reaching to the placenta.

关 键 词：子痫前期 维生素C 氧化应激 依赖钠离子维生素C转运蛋白 

分 类 号：R714.24[医药卫生—妇产科学]