LFA-1缺失诱导小鼠的CD3+细胞上调  

Lacking LFA-1 Induced the Increasing of CD3+ Cells in Mice

作　　者：李江超[1] 张潇涵[1] 刘擎[1] 何晓东[1] 周泽启 叶宇翔[1] 王丽京[1] 

机构地区：[1]广东药学院血管生物研究所,广州510006

出　　处：《基因组学与应用生物学》2016年第3期507-512,共6页Genomics and Applied Biology

基　　金：国家自然基金(No.81472336和No.31471290);广东省医学科研基金(B2014207);广东省科技计划(2015A030302086和2014A020212313)共同资助

摘　　要：淋巴细胞功能相关抗原-1(lymphocyte function-associated antigen 1,LFA-1)是白细胞上的重要粘附分子。本研究旨在探讨LFA-1缺失(LFA-1^(-/-))后小鼠血液、骨髓、脾脏中血液细胞分类和T细胞亚群的变化。本研究采用LFA-1^(-/-)小鼠转基因小鼠,经PCR鉴定其基因型;采用全自动血液检测仪测定血液细胞分类。为进一步了解淋巴细胞亚群的变化,采用流式细胞仪检测LFA-1^(-/-)和对照组小鼠血液、骨髓、和脾脏中T淋巴细胞亚群。结果表明,LFA-1^(-/-)小鼠血液中白细胞总数增多;在骨髓,血液和脾脏中CD3+T淋巴细胞增多。根据研究结果推测LFA-1可能调控CD3+细胞的分化。The Lymphocyte function associated antigen 1（lymphocyte function-associated antigen 1,LFA-1） is an important adhesion molecule on the white blood cells. We explored the change of blood cells in its classifications and numbers in blood,bone marrow and spleen of LFA-1^（-/-）mice. We used LFA-1^（-/-）mice as the material,and indentified LFA-1 genotype by PCR. Automatic blood counter system were used to measure white blood cell classification. In order to further understand the changes of lymphocyte subsets,flow cytometry instrument was used to detect T lymphocytes subtype in blood,bone marrow and spleen. The results showed that the total number of white blood cells in the blood was increased in LFA-1^（-/-）mice,and CD3＋ lymphocytes was increased in bone marrow and spleen. The results speculated that LFA-1 might regulate the differentiation of CD3＋ cells.

关 键 词：LFA-1 白细胞 T淋巴细胞 基因工程鼠 

分 类 号：R392[医药卫生—免疫学]