红霉素对胃食管反流幼兔模型胃肠道动力作用的影响  

Effect of Erythromycin on gastrointestinal motile function in young rabbits models with gastroesophageal reflux

作　　者：高颜 王春晖 林燕 陈鹏德 江逊 王宝西 

机构地区：[1]第四军医大学第二附属医院儿科,西安710038

出　　处：《中华实用儿科临床杂志》2016年第7期491-495,共5页Chinese Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金（81370490,81170331）

摘　　要：目的 建立胃食管反流（GER）幼兔模型，观察红霉素对模型幼兔肌条不同部位（食管下段和胃底）的影响以及损毁cajal间质细胞（ICC）后红霉素对肌条的影响。方法8周龄的幼兔40只随机分为对照组（20只）和实验组（20只），实验组通过部分胃底结扎联合幽门限制法建立GER的幼兔模型，对照组分离胃底和幽门后即关腹。4周后随机取实验组和对照组幼兔各10只，每只取食管下端和胃底肌条各2条，取其中1条进行ICC的损毁，离体肌条组织电生理检测模型食管下段和胃底肌条的频率和振幅变化。结果1．处理后4周通过pH梯度测定、形态学观察、HE染色鉴定GER模型建立成功，ICC的特异性受体c-kit蛋白的免疫组织化学染色显示实验组肌条ICC表达数量显著减少，形态发生变化。2．检测实验组与对照组食管下段和胃底肌条的振幅与频率，对照组食管下段的频率和振幅分别为（2．60±0．70）次／min和（3．03±0．59）g，实验组食管下段肌条的频率和振幅分别为（0．29±0．49）次／min和（0．61±0．43）g，2组比较差异均有统计学意义（t=9．86、8．15，P均〈0．05）。对照组胃底肌条的频率和振幅分别为（3．50±0．97）次／min和（5．33±1．09）g，实验组胃底肌条的频率和振幅分别为（0．43±0．54）次／min和（1．13±0．41）g，2组比较差异均有统计学意义（t=8．29、12．55，P均〈0．05）。3．红霉素加入后食管下段和胃底肌条的频率和振幅变化：实验组食管下段肌条振幅为（1．16±0．83）g，与加入药物前比较差异有统计学意义（t=2．35，P〈0．05），频率与加入药物前比较差异无统计学意义；胃底肌条的振幅增加至（4．20±1．14）g，与加入药物前比较差异有统计学意义（t=4．76，P〈0．05），频率与加入药物前比较差异无统计学意义。对照组食管下段肌条的频率和振幅为�Objective To establish the young rabbit models with gastroesophageal reflux（GER） and observe the effect of erythromycin in different parts of muscle segment （fundus ventriculi and lower esophageal） , so as to investigate the variation after destroying the interstitial cells of Cajal （ ICC ）. Methods Forty young rabbits of 8 weeks old were randomly divided into control group（ n = 20） and experimental group （n = 20）. In the experimental group, the fundus ventriculus was partly ligated combined with helicobacter restriction at a level near the pylorus to establish GER young rabbit models. In the control group, the fundus ventriculi and pylorus were dissociated then the abdomen was closed. Four weeks later, 10 rabbits from the experimental group and 10 rabbits from the control group were taken, 2 lower esophageal segments and 2 fundus ventriculi muscle segments were split in each animal, and the ICC were destroyed at 1 lower esophageal segment and 1 fundus ventriculi muscle segment. The amplitude and frequency of lower esophageal and fundus ventrieuli muscle segments were measured by using electropbysiology in vitro. Results （ 1 ） After 4 weeks of operation, the GER young rabbit models were successfully established. The data were detected by esophageal pH monitoring, general morphology observation and HE stain. Immunohistochemical staining of specific receptors protein c - kit on ICC showed that the ICC in the experimental group was reduced remarkably, and the morphological characteristics changed. （2） In the control group, the frequency and amplitude of lower esophageal muscle segments were （2.60 ± 0.70） times/min and （ 3.03 ±0.59 ） g, the frequency and amplitude of fundus ventriculi muscle segments were （ 3.50 ± 0.97 ） times/min and （ 5.33± 1.09 ） g ; in the experimental group, the frequency and amplitude of lower esophageal muscle segments were reduced to（0.29 ± 0.49）times/min and （0.61 ± 0.43） g;the frequency of fundus ventriculi muscle segmens in the expe

关 键 词：胃食管反流 动物模型 红霉素 CAJAL间质细胞 

分 类 号：R725.7[医药卫生—儿科] R-332[医药卫生—临床医学]