中药气阴双补对缓解期哮喘模型大鼠气道重塑及ERK信号分子表达的影响  

Effects of Chinese Medical Qi and Yin Method on Airway Remodeling and ERK Signal Molecule Expression in Asthmatic Rats

作　　者：周星星[1] 徐贤达 阮晓琳 宣桂琪[3] 陈健[3] 

机构地区：[1]浙江中医药大学第一临床医学院,杭州310053 [2]江苏省徐州市中医院儿科,徐州221000 [3]浙江中医药大学附属第一医院儿科,杭州310006

出　　处：《浙江中西医结合杂志》2016年第4期317-321,共5页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省自然科学基金资助项目(No.LY15H270004)

摘　　要：目的研究中药气阴双补对缓解期哮喘模型大鼠气道重塑及细胞外信号调节蛋白激酶(ERK)信号分子表达的影响。方法 6周龄健康雄性SD大鼠48只,随机分为正常组、模型组、中药低剂量组、中药中剂量组、中药高剂量组及地塞米松组,每组8只。用卵清蛋白(OVA)致敏和激发建立哮喘气道重塑大鼠模型,肺组织病理图像分析气道重塑,RT-PCR检测ERK、ERK2m RNA在肺组织表达,免疫印迹检测P-ERK蛋白在气道平滑肌表达。结果模型组肺组织切片平滑肌标化面积(WAm/Pbm)为(17.34±0.63)μm^2/μm、内管壁标化面积(WAi/Pbm)为(42.35±3.31)μm^2/μm,显著高于正常组的(7.63±0.32)μm^2/μm和(26.73±1.50)μm^2/μm(P<0.05);中药低剂量组WAm/Pbm为(13.68±0.52)μm^2/μm,WAi/Pbm为(37.53±2.57)μm^2/μm,中剂量组分别为(10.75±0.72)μm^2/μm、(33.74±1.21)μm^2/μm,高剂量组分别为(9.33±0.92)μm^2/μm、(30.62±1.34)μm^2/μm,均较模型组显著降低(P<0.05),但仍高于正常组(P<0.05);模型组ERK1mRNA(4.26±0.52)、ERK2mRNA(3.54±0.37)、p-ERK(3.288±0.641)表达均显著高于正常组(1.02±0.11、0.96±0.13、0.703±0.338,P<0.05);中药低剂量组ERK1mRNA(3.23±0.45)、ERK2mRNA(2.72±0.21),中剂量组ERK1mRNA(1.65±0.26)、ERK2mRNA(1.36±0.14)、p-ERK(2.172±0.221),中药高剂量组ERK1mRNA(2.37±0.23)、ERK2mRNA(2.03±0.12)、p-ERK(1.127±0.631)均明显低于模型组(P<0.05),但仍高于正常组(P<0.05)。结论中药气阴双补可改善缓解期哮喘大鼠气道重塑,抑制ERKl/2m RNA及P-ERKl/2蛋白在气道中的表达。Objective To investigate the influence of traditional Chinese medicine Qi-Yin method on trachea remodeling and the expression of extracellular signal adjust the protein kinase（ERK） signal molecules in stable asthmatic rats. Methods Forty-eight weight 180-200 g healthy male Wistar rats were randomly divided into blank control group, asthma model group, traditional Chinese medicine low-dose group, traditional Chinese medicine middle-dose group, traditional Chinese medicine high-dose groupand dexamethasone group, with 8 rats in each group.The asthma trachea remodeling rat model was set up with ovalbumin（OVA） sensitization and activation. Lung tissue pathology image analysis was used to analyze trachea remodeling, RT-PCR to detect ERK1/2m RNA expression in trachea, western blot to determine p-ERK protein expression in trachea smooth muscle. Results Compared with normal group, asthma model group had higher lung tissue section smooth muscle standardized area（WAm/Pbm） and inside tube wall standardized area（WAi/Pbm）（17.34±0.63μm-2/μm vs 7.63±0.32μm-2/μm; 42.35±3.31μm-2/μm vs26.73±1.50μm-2/μm; all P〈0.05）. The WAm/Pbm and WAi/Pbm were 13.68±0.52μm-2/μm and 37.53±2.57μm-2/μm in traditional Chinese medicine low-dose group, 10.57 ±0.72μm-2/μm and 33.74 ±1.21μm-2/μm in traditional Chinese medicine middle-dose group, 9.33 ±0.92μm-2/μm and 30.62 ±1.34μm-2/μm in traditional Chinese medicine high-dose group, which were significantly reduced compared to asthma model group, but still higher than those in blank control group（all P〈0.05）. ERK1 m RNA, ERK2 m RNA, and p-ERK protein expression were significantly higher in asthma model group than those in blank control group（4.26±0.52, 3.54±0.37, 3.288±0.641 vs 1.02±0.11, 0.96±0.13,0.703±0.338; all P〈0.05）. The ERK1/2m RNA in traditional Chinese medicine low-dose group were 3.23±0.45 and2.72±0.21, in traditional Chinese medicine middle-dose group were 1.65±0.26 and 1.36±0.14, in traditional Chinese medicine high-do

关 键 词：大鼠 哮喘 气道重塑 细胞外信号调节蛋白激酶(ERK) 气阴双补 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]