不同产地和品种天麻的HPLC指纹图谱研究  

HPLC fingerprint of the different producing areas and different varieties of Gastrodia elata

作　　者：杨芝芳[1] 邓薇[1] 宗露[1,2] 徐大东 李振斌[1] 蒋运斌[1] 马逾英[1] 

机构地区：[1]成都中医药大学中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137 [2]四川省食品药品检验检测院,四川成都610079 [3]四川平武县生产力促进中心,四川平武622550

出　　处：《华西药学杂志》2016年第2期188-191,共4页West China Journal of Pharmaceutical Sciences

基　　金：四川省科技富民强县专项计划(编号:2015);成都中医药大学自然科学基金课题(编号:030021039)

摘　　要：目的研究不同产地和不同品种天麻的HPLC指纹图谱。方法色谱柱为Diamonil C18柱（250 mm×4.6 mm,5μm）,流动相为甲醇-0.1%磷酸水溶液,梯度洗脱,检测波长220 nm,流速1 m L·min-1,柱温35℃。对20批天麻样品的HPLC指纹图谱进行相似度评价和聚类分析。结果确立了13个共有峰,建立了天麻药材HPLC指纹图谱共有峰模式。20批不同产地和品种天麻的相似度为0.829~1.000,综合评判得分为-0.77~1.07。结论建立的HPLC指纹图谱反映了天麻药材中的化学成分信息,同一产地天麻的化学成分趋同,而不同产地的天麻差异较大,表明产地因素对其化学成分的影响较大。OBJECTIVE To study the fingerprint of the different origins and different varieties of Gastrodia elata. METHODS The HPLC separation was performed on a Diamonsil C18（ 250 mm × 4. 6 mm,5 μm） analytical column gradient eluted with methanol-0. 1% phosphoric acid water at the flow rate of 1. 0 m L·min- 1. The UV detection wavelength was 220 nm and the temperature of column was 35 ℃. The similarity evaluation and cluster analysis were used to analyse the 20 batches of samples. RESULTS HPLC reference fingerprint of G. elata was established with thirteen common peaks. The similarity was between 0. 829- 1. 000 of the twenty batches of G. elata from the different areas and different varieties,and the comprehensive evaluation scores was between 0. 77- 1. 07.CONCLUSION The established HPLC fingerprint reflected the information on the chemical composition of G. elata. The chemical constituents of G. elata from the same origin are the same,but the G. elata from the different origins are not the same. It shows that the factor of producing area has a great impact on the chemical composition.

关 键 词：天麻 HPLC 指纹图谱 相似度评价 聚类分析 化学成分 

分 类 号：R917[医药卫生—药物分析学]