UPLC测定红毛五加嫩叶中的绿原酸  

Determination of chlorogenic acid from Acanthopanacis giraldii tender leaves by UPLC

作　　者：陈奕君[1] 赵薇[1] 翟雪[1] 刘春林[1] 刘圆[2] 

机构地区：[1]西南民族大学化学与环境保护工程学院,四川成都610041 [2]西南民族大学民族医药研究院,四川成都610041

出　　处：《华西药学杂志》2016年第2期199-201,共3页West China Journal of Pharmaceutical Sciences

基　　金：国家自然科学基金资助项目(批准号:81173653);西南民族大学2016年研究生创新型科研项目(CX2016SZ014)

摘　　要：目的采用UPLC测定红毛五加嫩叶试制品中绿原酸的含量并比较差异。方法 Acquity UPLCBEH C18柱（50 mm×2.1 mm,1.7μm）,柱温30℃,流动相为乙腈-2‰磷酸溶液（10∶90）,检测波长327 nm,流速0.2 m L·min^-1。结果绿原酸0.02~0.72 mg·m L^-1与峰面积具有良好的线性关系,回归方程为：Y=3×10^7X-9.9481×10^4（r2=0.9997,n=9）,平均回收率为97.7%,RSD=0.61%（n=6）,红毛五加嫩叶晒干品中绿原酸的含量最高（0.0326 g·g^-1）,百合粉中绿原酸含量为0。结论所用方法简便、准确、重复性好,能为红毛五加嫩叶的开发提供参考。OBJECTIVE To establish a UPLC method to determine the content of chlorgenic acid from Acanthopanacis giraldii tender leaves and compare the different contents. METHODS The analysis was carried out at 30 ℃ on an ACQUITY UPLC BEH C18（ 50 mm × 2. 1 mm,1. 7 μm） column eluted with a mobile phase consisted of acetonitrile（ A）- water with 2‰ phosphoric acid（ B）（ 10∶90）. The detective wavelength was 327 nm with the flow rate of 0. 2 m L·min^-1. RESULTS The calibration curve of chlorogenic acid was linear within the concentration range of 0. 02 mg·m L^-1to 0. 72 mg·m L^-1with r2 of 0. 9997（ n = 9）. The average recovery was97. 7% with the RSD of 0. 61%（ n = 6）. The tender leaves dried by sun contained the highest content of chlorogenic acid and per gram of crude drug contained 0. 0326 g of chlorogenic acid. CONCLUSION This method is simple,accurate and repeatable. The experi-mental results can provide useful data for the development of tender leaves products.

关 键 词：红毛五加 嫩叶 绿原酸 超高效液相色谱 含量测定 

分 类 号：R917[医药卫生—药物分析学]