HPLC测定伊赫乌兰-13中的大叶茜草素  

Determination of mollugin in Yihewulan-13 by HPLC

作　　者：邹继红[1] 王洪斌 赵春杰[3] 

机构地区：[1]赤峰学院医学院,内蒙古赤峰024000 [2]赤峰市传染病防治医院,内蒙古赤峰024000 [3]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《华西药学杂志》2016年第2期208-209,共2页West China Journal of Pharmaceutical Sciences

基　　金：内蒙古自治区自然科学基金资助项目(2013MS1217)

摘　　要：目的采用HPLC法测定伊赫乌兰-13中大叶茜草素的含量。方法色谱柱为Agilent Extend-C18,流动相为甲醇-水（94∶6）,流速1.0 m L·min^-1,检测波长250 nm。结果回归方程为：Y=90.416X＋26.478（r=0.9998）,大叶茜草素4.28~21.4 mg·L^-1与峰面积的线性关系良好;平均加样回收率为99.3%,RSD=1.4%（n=9）。结论所用方法快速、简便、准确,适用于伊赫乌兰-13中大叶茜草素的质量控制。OBJECTIVE To establish an HPLC method for the determination of mollugin in Yihewulan^-13. METHODS Seperation was achieved on an Agilent Extend- C18 column and detected wavelength was 250 nm. The mobile phase was the mixture of methanol and water（ 94 ∶6） at a flow rate of 1. 0 m L·min^-1. RESULTS The regression equation was Y = 90. 416 X ＋ 26. 478（ r =0. 9998）,and the linear range of mollugin was 4. 28- 21. 4 mg·L^-1. The average recovery was 99. 4% with the RSD of 1. 4%（ n = 9）.CONCLUSION The method is quick,simple,accurate,and suitable for the determination of mollugin in Yihewulan^-13.

关 键 词：高效液相色谱法 伊赫乌兰-13 大叶茜草素 含量测定 

分 类 号：R917[医药卫生—药物分析学]