盐诱导高血压大鼠左心室间质胶原重构及白细胞介素17A、肿瘤坏死因子α、转化生长因子β_1和基质金属蛋白酶9的表达  

The remodeling of ventricular interstitial collagen and expression of interleukin 17A,tumor necrosis factor-α,transforming growth factor-β_1 and matrix metalloproteinase-9 in salt-induced hypertensive rat model

作　　者：陈真[1] 黄荣杰[1] 李健玲[1] 

机构地区：[1]广西医科大学第一附属医院高血压病区,广西南宁530021

出　　处：《中华高血压杂志》2016年第2期152-157,共6页Chinese Journal of Hypertension

基　　金：广西自然科学基金(2012GXNSFAA239004)

摘　　要：目的探讨盐诱导高血压大鼠模型白细胞介素17A(IL-17A)、肿瘤坏死因子α(TNF-α)、转化生长因子β_1(TGF-β_1)和基质金属蛋白酶(MMP)9的表达以及IL-17A在该模型大鼠左心室间质胶原重构中的作用。方法将48只5周龄Wistar大鼠,随机分为正常对照组、2%盐浓度组和3%盐浓度组各16只,分别用蒸馏水、2%或3%NaCl饲养10周后,尾袖法测尾动脉血压,处死取左心室心肌组织,HE染色观察心肌细胞形态,Masson染色观察心肌胶原纤维并计算胶原容积分数(CVF),酶联免疫吸附试验(ELISA)、免疫组化法分别检测血浆和心肌组织IL-17A、TNF-α、MMP-9和TGF-β_1表达。结果2%盐浓度组高血压成模率75.00%(12/16),死亡率6.25%(1/16),3%盐浓度组成模率81.25%(13/16),死亡率12.50%(2/16);饲养10周后2%盐浓度组和3%盐浓度组大鼠的收缩压与对照组相比明显增高[(182.1±20.2)、(183.3±31.7)比(147.1±10.9)mm Hg,均P<0.01],但2%盐浓度组和3%盐浓度组两组间差异无统计学意义。2%盐浓度组和3%盐浓度组大鼠血浆和左心室心肌的IL-17A、TNF-α、MMP-9和TGF-β_1表达增高。IL-17A与CVF、MMP-9、TNF-α、TGF-β_1表达呈正相关(r=0.3、0.4、0.5、0.5,均P<0.05);CVF与TNF-α、TGF-β_1、MMP-9呈正相关(r=0.6、0.7、0.8;均P<0.01)。结论盐诱导高血压大鼠血浆和左心室心肌IL-17A表达明显增高,与TNF-α、MMP-9、TGF-β_1表达呈正相关。Objective To investigate the expression of interleukin-17A（IL-17A）,tumor necrosis factor-α（TNF-α）,transforming growth factor-β_1（TGF-β_1）and matrix metalloproteinase-9（MMP9）in salt-induced hypertensive rat model,and the role of IL-17 Ain the left ventricular collagen remodeling in this model. Methods Forty-eight5-week-old Wistar rats were randomly divided into three groups,with 16 in each group：control group（drinking distilled water）,2%salinity group（drinking 2%sodium chloride solution）and 3%salinity group（drinking 3%sodium chloride solution）. After 10 weeks of intervention,systolic blood pressure（SBP）was measured by tail-cuff technique,myocardial morphological and structural changes were observed by hematoxylin-eosin staining（HE）,myocardial collagen fibers were observed and collagen volume fraction（CVF）was calculated by Masson staining.The levels of TNF-α,TGF-β_1,IL-17 Aand MMP-9in left ventricle and blood plasma were determined by immunohistochemistry and enzyme linked immunsorbent assay（ELISA）respectively. Results The incidences of hypertension were 75.00%（12/16）and 81.25%（13/16）,and death rates were 6.25%（1/16）and 12.50%（2/16）,respectively in 2% salinity group and 3%salinity group. After 10-weeks salinity intervention,SBP in 2%and 3%salinity-treated rats increased significantly compared to normal control group. But no significant difference was found between the 2% salinity and 3% salinity-treated groups. The levels of IL-17 A,TNF-α,TGF-β_1and MMP-9in left ventricle and blood plasma of 2% and 3% salinity-treated rats were higher than the normal control group. IL-17 A was positively correlated with CVF,MMP-9,TNF-αand TGF-β_1（r=0.3,0.4,0.5,0.5;all P0.05）;Meanwhile,CVF was positively related to TNF-α,TGF-β_1and MMP-9（r=0.6,0.7,0.8;all P0.01）. Conclusion IL-17 Aexpression is significantly increased in left ventricular myocardium and plasma of salt-induced hypertensive rat. The expression of IL-17 Ais positively correlated with the T

关 键 词：白细胞介素17A 盐 高血压 心肌间质重构 

分 类 号：R544.1[医药卫生—心血管疾病]