Genetic Diversity Analysis on Rep-PCR Genomic Fingerprinting and 16S rDNA Sequences of Desulfurization Bacteria  

作　　者：吕英海 祝加伟 张雨晴 蔡晓青 郭凯 周仕学 

机构地区：[1]College of Chemical and Environmental Engineering,Shandong University of Science and Technology

出　　处：《Journal of Donghua University(English Edition)》2016年第1期134-137,共4页东华大学学报（英文版）

基　　金：National Natural Science Foundations of China(Nos.41302079,21176145);Project of Shandong Province Higher Educational Science and Technology Program,China(No.J13LD54)

摘　　要：In order to reduce deleterious effect on environment,human health and facilities caused by original sulfides, more attention should be paid to biodesulfurization studying for fossil fuels. In this work, eight isolates were characterized by several DNA-based methods such as BOX element polymerase chain reaction( BOX-PCR), enterobacterial repetitive intergenic consensus( ERIC)-PCR and random amplification of polymorphic DNA( RAPD)-PCR. The desulfurization performance was determined by micro-coulometric method,Gibb's assay and barium sulfate test. It was found out that ERIC-PCR displays a much higher inter-strain heterogeneity compared with using BOX. The length of the primer didnot play the most important role in bacterial classification. The combination of the analysis of repetitive-sequence-based polymerase chain reaction ngerprinting and 16 S r DNA was able to provide more effective way in the separation and identification of bacteria.According to the analysis of 16 S r DNA,the more efficient desulfurization strain should belong to Klebsiella variicola.In order to reduce deleterious effect on environment,human health and facilities caused by original sulfides, more attention should be paid to biodesulfurization studying for fossil fuels. In this work, eight isolates were characterized by several DNA-based methods such as BOX element polymerase chain reaction（ BOX-PCR）, enterobacterial repetitive intergenic consensus（ ERIC）-PCR and random amplification of polymorphic DNA（ RAPD）-PCR. The desulfurization performance was determined by micro-coulometric method,Gibb＇s assay and barium sulfate test. It was found out that ERIC-PCR displays a much higher inter-strain heterogeneity compared with using BOX. The length of the primer didnot play the most important role in bacterial classification. The combination of the analysis of repetitive-sequence-based polymerase chain reaction ngerprinting and 16 S r DNA was able to provide more effective way in the separation and identification of bacteria.According to the analysis of 16 S r DNA,the more efficient desulfurization strain should belong to Klebsiella variicola.

关 键 词：16S rDNA BOX element polymerase chain reaction(BOX-PCR) DESULFURIZATION fingerprinting enterobacterial repetitive intergenic consensus(ERIC)-PCR 

分 类 号：Q93-331[生物学—微生物学]