铁倍蚜属枣铁亚种mtDNA COI基因序列遗传分化  

Population Genetic Differentiation of Kaburagia rhusicola ensigallis Inferred from mtDNA COI Sequences

作　　者：李京隆[1] 任竹梅[1] 

机构地区：[1]山西大学生命科学学院,山西太原030006

出　　处：《山西农业科学》2016年第4期432-435,共4页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31170359);国家"863"主题项目(SS2014AA021802)

摘　　要：测定铁倍蚜属枣铁亚种7个种群共58个个体的mt DNA COI基因部分序列,分析其种群遗传结构和变异。结果表明,在测得的1 257 bp COI基因序列中有100个变异位点,58条序列共产生23个单倍型,其平均单倍型多样度为0.943±0.015,核苷酸多样度为0.020±0.002,其中,4个单倍型为不同种群的共享单倍型(2个单倍型为主体单倍型),11个单倍型为独享单倍型,8个单倍型为同一种群不同个体共享。AMOVA分析显示,枣铁亚种种群间的遗传变异较高,且分化显著。TCS网络图和聚类关系综合分析显示,不同单倍型按地理分布形成较明显的簇群,其中,云南水富所有个体单独构成一个分支,与其余种群关系较远,该种群可能属于不同的种。The population genetic diversity and structure of the Rhus gall aphid Kaburagia rhusicola ensigallis were examined by analyzingthe DNA sequencesofthemitochondrialcytochromecoxidasesubunits I. The1 257 bp sequenceswere obtained from 58 individuals of seven populations, among which there were 100 variable sites, accounting for about 7.9% of the total measured nucleotide sequence. A total of 23 haplotypes were detected based on 58 individuals across seven populations, among which there were two main haplotypes.There were eleven haplotypes shared by only one individual from one population, and eight haplotypes were shared by the different individuals from the same population, respectively. The average diversity of haplotypes was 0.943±0.015, and the average nucleotide diversity was 0.020 ±0.002. The molecular variance analysis（AMOVA） showed that the population genetic differentiation in K.rhuisicola ensigallis populations was very significant. The MP phylogenetic trees and TCS network relationship suggested that there was significant difference among different populations of K. rhusicola ensigallis, especially between the SF population and the others, and which might be a different species.

关 键 词：铁倍蚜属枣铁亚种 COI基因 DNA序列 遗传分化 

分 类 号：S899.4[农业科学—特种经济动物饲养]