Effect of Cold Plasma on Cell Viability and Collagen Synthesis in Cultured Murine Fibroblasts  

作　　者：石兴民 蔡晶芬 许桂敏 任鸿斌 陈思乐 常正实 刘进仁 黄崇亚 张冠军 吴喜利 

机构地区：[1]School of Public Health,Medical Science Center,Xi'an Jiaotong University [2]State Key Laboratory of Electrical Insulation and Power Equipment,School of Electrical Engineering,Xi'an Jiaotong University [3]Second Affiliated Hospital,Xi'an Jiaotong University

出　　处：《Plasma Science and Technology》2016年第4期353-359,共7页等离子体科学和技术（英文版）

基　　金：supported partly by National Natural Science Foundation of China(Nos.81372076,51307133 and 51221005);China National Funds for Distinguished Young Scientists(No.51125029);the Sci-Tech Project of Shaanxi Province of China(No.2010K16-04);the Fundamental Research Funds for the Central Universities of China(No.xkjc2013004)

摘　　要：An argon atmospheric pressure plasma jet was employed to treat L929 murine fibroblasts cultured in vitro.Experimental results showed that,compared with the control cells,the treatment of fibroblasts with 15 s of plasma led to a significant increase of cell viability and collagen synthesis,while the treatment of 25 s plasma resulted in a remarkable decrease.Exploration of related mechanisms suggested that cold plasma could up-regulate Cyclin D1 gene expression and down-regulate p27 gene expression at a low dose,while it could down-regulate Cyclin D1 expression and up-regulate p27 expression at a higher dose,thus altering the cell cycle progression,and then affecting cell viability and collagen synthesis of fibroblasts.An argon atmospheric pressure plasma jet was employed to treat L929 murine fibroblasts cultured in vitro.Experimental results showed that,compared with the control cells,the treatment of fibroblasts with 15 s of plasma led to a significant increase of cell viability and collagen synthesis,while the treatment of 25 s plasma resulted in a remarkable decrease.Exploration of related mechanisms suggested that cold plasma could up-regulate Cyclin D1 gene expression and down-regulate p27 gene expression at a low dose,while it could down-regulate Cyclin D1 expression and up-regulate p27 expression at a higher dose,thus altering the cell cycle progression,and then affecting cell viability and collagen synthesis of fibroblasts.

关 键 词：cold plasma cell viability collagen synthesis fibroblasts 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]