RP-HPLC法同时测定人血清中拉莫三嗪、苯巴比妥、奥卡西平及其活性代谢物单羟基卡马西平的浓度  

Determination of Lamotrigine,Phenobarbital,Oxcarbazepine and Its Active Metabolite Monhydroxycarbazepin Concentrations in Human Serum by RP-HPLC

作　　者：沈芊[1] 王彦改[1] 曾红[1] 王雪梅[1] 闫素英[1] 

机构地区：[1]首都医科大学宣武医院药剂科,北京100053

出　　处：《中国药房》2016年第11期1493-1496,共4页China Pharmacy

摘　　要：目的：建立同时测定人血清中拉莫三嗪（LTG）、苯巴比妥（PBB）、奥卡西平（OXC）及其活性代谢物单羟基卡马西平（MHD）浓度的方法。方法：血清采用二氯甲烷液-液萃取法处理后以反相高效液相色谱（RP-HPLC）法进样测定,色谱柱为Kromasil 100-C18,流动相为水相（由0.5%三氟乙酸与14 mmol/L甲酸铵溶液按2∶1体积比混合）-乙腈（76∶24,V/V）,流速为1.0 ml/min,柱温为常温,紫外检测波长为215 nm。结果：MHD、LTG、PBB和OXC血药浓度分别在0.40-80.35、0.41-81.10、0.45-90.00、0.39-78.80μg/ml范围内线性关系良好（r均〉0.999 3,n=7）;提取回收率为54.09%-83.78%,方法回收率为93.83%-113.25%,各待测组分的日内、日间RSD均≤13.51%。其他常用药物不影响待测组分的测定。结论：本方法准确、灵敏、特异性强、测定时间短,可满足临床常规治疗药物监测的需要。OBJECTIVE：To establish a method for simultaneous determination of lamotrigine（LTG）,Phenobarbital（PBB）,oxcarbazepine（OXC） and its active metabolite monohydroxycarbazepine（MHD） in human serum. METHODS：Serum samples were extracted with dichloromethane by liquid-liquid extraction,and then determined by RP-HPLC. Kromasil 100-C18 column was used with mobile phase consisted of acetonitrile（0.5% TFA and 14 mmol/L formic acid amine solution,2 ∶ 1,V/V）-acetonitrile（76 ∶24,V/V）at flow rate of 1.0 ml/min. The column temperature was room temperature,and detection wavelength was set at 215 nm.RESULTS：The liner range of MHD,LTG,PBB and OXC were 0.40-80.35,0.41-81.10,0.45-90.00 and 0.39-78.80 μg/ml,respectively（all r〉0.999 3,n=7）. The extraction recovery was 54.09%-83.78%,and method recovery was 93.83%-113.25%. Both inter-day and intra-day RSDs were less than 13.51%. Other commonly used drugs didn＇t affect the determination of component to be measured. CONCLUSIONS：The method is accurate,sensitive and specific,and spend less time. It can meet the demand of clinical drug monitoring.

关 键 词：拉莫三嗪 奥卡西平 苯巴比妥 单羟基卡马西平 血药浓度 反相高效液相色谱法 

分 类 号：R969.1[医药卫生—药理学]