云南泸定百合8个野生居群的染色体核型分析  

Karyotypes Analysis of Eight Wild Populations of Lilium sargentiae from Yunnan Provice

作　　者：段青[1] 崔光芬[1] 贾文杰[1] 马璐琳[1] 王继华[1] 杜文文[1] 王祥宁[1] 

机构地区：[1]云南省农业科学院花卉研究所,云南省花卉育种重点实验室,云南省花卉工程技术研究中心,国家观赏园艺工程技术研究中心,昆明650205

出　　处：《西北植物学报》2016年第3期472-478,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家‘863’计划(2011AA100208);云南省应用基础研究计划青年项目(2012FD072);云南省科技计划项目(2012BB011)

摘　　要：用常规压片法,对云南省境内泸定百合（Lilium sargentiae Wilson）8个野生居群的染色体进行了核型分析,结果显示：（1）野生泸定百合8个居群均为二倍体（2n=2x=24）,染色体基数为12,共24条染色体,无B染色体;（2）核型类型均为3A,第1、2对染色体均为具中部（m）或近中部（sm）着丝点的大形染色体,且第1对染色体上均具有随体;（3）染色体长度比为1.64~2.26,平均臂比为6.43~8.22,核型不对称系数为79.82%~81.77%。研究表明,泸定百合染色体数相对稳定,染色体变异主要表现在结构变异,各个居群的染色体组成、随体数、随体位置、臂比、染色体长度比及核型不对称系数均有差异,泸定百合各居群间存在明显的核型多态性。Karyological study of 8wild populations of Lilium sargentiae Wilson from Yunnan Province was carried out by traditional squashing and pressing method.The results were the following：（1）the base number of chromosome in L.sargentiae was 12（x=12）.All of the 8wild populations were diploid（2n=2x=24）and without B chromosome;（2）karyotypes of 8 wild populations of L.sargentiae were ‘3A＇,Their first and second pairs of chromosomes were either large median chromosomes（m）or large submedian chromosomes（sm）.All the first pairs of chromosomes of the 8populations had satellite chromosomes;（3）chromosome length ratio were from 1.64 to 2.26,the mean arm ratio ranged from 6.43 to 8.22,and the karyotype asymmetry coefficient（As.K.%）of the 8populations were from 79.82%to 81.77%.The above results indicated that the chromosome number of L.sargentiae was stable,and the chromosome variation among populations was generally coming from structure variation.The 8populations had obvious differences in the composition of chromosomes,the number and distribution of satellite,chromosomes arm ratio,chromosome length ratio and As.K.%.There were obvious karyological variations among populations of L.sargentiae.

关 键 词：泸定百合 居群 染色体 核型 

分 类 号：Q343.22[生物学—遗传学]