尼罗红荧光染色法的优化及应用  

The Optimization and Application of Nile Red Fluorescent Determination

作　　者：石健[1] 张雯婕[1] 冯汛 胡雨婷[1] 瑶池[1] 

机构地区：[1]南通大学,江苏南通226019

出　　处：《工业安全与环保》2016年第4期61-64,共4页Industrial Safety and Environmental Protection

基　　金：国家自然科学基金(21177067);江苏省自然科学基金重点项目(BK2010034);江苏省基础研究基金(BK2011009);南通市社会发展项目(HS2012001);南通市重点实验室项目(CP12011001);江苏省青蓝工程计划项目

摘　　要：以自主筛选的小球藻为材料对尼罗红荧光染色进行条件优化,并用该方法追踪微藻在污水中的油脂含量变化。实验发现,微藻只有经过超声波破碎,染色才会出现荧光,DMSO的添加会增强荧光反应。最终确定其染色条件为:DMSO与藻液体积比为1∶5,超声波破碎5 min后染色5 min,NR质量浓度1.5μg/m L。在其应用方面发现,微藻的油脂积累量只有培养基的一半,说明污水不利于微藻油脂的积累;最佳采收时期为平稳期,此时微藻的产量和油脂含量达到最高。The chlorella is screened as material to optimize Nile red fluorescent measure, and it is used to trance the varia- tion on microalgae oil in sewage. The experiment finds out that the dye won＇t work until microalgae is broken by ultrasonic, and the addition of DMSO will enhance fluorescence reaction. The optimization result is determined： VDMSO： Vsewage = 1：5,5 min broke cell by ultrasonic and dye 5rain, NR 1. 5μg/mL. As to the application, microalgae oil accumulation is only half compared to medium, indicating that sewage is not conducive to microalgae oil accumulation, and the best harvesting time is stationary phase, when the yield and oil content reaches the highest of micro algae.

关 键 词：小球藻 尼罗红 超声波 污水 油脂 

分 类 号：X703[环境科学与工程—环境工程]