移植干细胞死活活体示踪技术研究进展  

Progress of tracking the viability of transplanted stem cells in vivo

作　　者：林素颖 陈光村[2] 黄德华[1,2] 孟春[1] 王强斌[2] 

机构地区：[1]福州大学生物科学与工程学院,福州350116 [2]中国科学院苏州纳米技术与纳米仿生研究所,纳米生物医学部/国际实验室,中国科学院纳米-生物界面重点实验室,苏州215123

出　　处：《科学通报》2016年第10期1075-1085,共11页Chinese Science Bulletin

基　　金：中国科学院战略性科技先导专项(XDA01030200);国家自然科学基金(21303249;21425103;81401464;21501192)资助

摘　　要：近年来,干细胞再生医学作为未来治愈人类重大疾病最具潜力的方法之一,吸引了科学工作者的广泛关注.清晰了解移植干细胞在活体中的存活、迁移和分化行为是指导干细胞有效治疗的重要前提,其中移植干细胞在体内的存活率更是决定干细胞疗法成败的关键因素.因此,发展有效示踪干细胞死活的影像技术以有效评估干细胞在活体的存活,已成为当今的一大研究热点.本文就目前干细胞死活示踪活体影像技术的研究进展予以综述和展望.In the last decades, stem cell-based regenerative medicine has attracted intense attention and extraordinary expectation due to its potentials in the treatment of numerous major diseases, such as hepatic, cardiac, pulmonary, renal and neurological diseases. Clearly knowing the viability, distribution and differentiation of the transplanted stem cells in vivo is a prerequisite for better understanding the role of stem cells playing in the therapeutic process, in which the survival report of the transplanted stem cells in vivo is particularly crucial in determining the success of stem cell-based regenerative medicine. Therefore, the development of non-invasive imaging methods that can in situ monitor the viability of the transplanted stem cells is urgently needed. In this review, we summarize the recent progress in tracking the viability of the transplanted stem cells in vivo, including reporter-gene based methods, exogenous contrast label-based methods and multimodel imaging methods. The reporter-gene based methods rely on functional proteins that produce only in live cells, thus the imaging signals are specifically coresponding to the viability of cells. Reporter gene-based imagings, including the luciferase-based bioluminescence imaging（BLI）, ferritin-based magnetic resonance imaging（MRI） and thymidine kinase-based positron emission computed tomography（PET） have been the most robust technique for short- and long-term monitoring cell viability in vivo. Herein, we explain basic principles of these reporter gene-based methods, and describe current examples and future prospects of these methods. In contrast to reporter-gene based methods, methods using exogenous contrast labels such as quantum dots and superparamgnetic iron oxides give a strong signal in cell tracking, but they cannot assess cell survival or death for the agents continue to display signals when the transplanted stem cells are dying. More recently, new nanotechnologies using exogenous contrast labels were developed to monitor cell viabili

关 键 词：移植干细胞 存活 活体示踪 影像技术 

分 类 号：R457.7[医药卫生—治疗学]