Super-resolution imaging and tracking of TGF-β receptor II on living cells  

作　　者：Zi Ye Nan Li Libo Zhao Yahong Sun Hefei Ruan Mingliang Zhang Jinghe Yuan Xiaohong Fang 

机构地区：[1]Key Laboratory of Molecular Nanostructure and Nanotechnology, Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences

出　　处：《Science Bulletin》2016年第8期632-638,共7页科学通报（英文版）

基　　金：supported by the National Basic Research Program of China(2013CB933701);the National Natural Science Foundation of China(21127901;91413119;91213305);the Chinese Academy of Science

摘　　要：Single-particle tracking photoactivated local- ization microscopy （sptPALM） has recently emerged as a powerful tool for high-density imaging and tracking of individual molecules in living cells. In this work, we have monitored and compared the diffusion dynamics of TGF-β type II receptor （TβRII） at high expression level using both traditional single-particle tracking （SPT） and sptPALM. The ligand-induced aggregation of TβRII oligomers was further indicated by sptPALM. Due to the capacity of distinguishing and tracking single molecules within diffraction limit, sptPALM outperforms traditional SPT by providing more accurate biophysical information,Single-particle tracking photoactivated localization microscopy(spt PALM) has recently emerged as a powerful tool for high-density imaging and tracking of individual molecules in living cells. In this work, we have monitored and compared the diffusion dynamics of TGF-b type II receptor(Tb RII) at high expression level using both traditional single-particle tracking(SPT) and spt PALM.The ligand-induced aggregation of Tb RII oligomers was further indicated by spt PALM. Due to the capacity of distinguishing and tracking single molecules within diffraction limit, spt PALM outperforms traditional SPT by providing more accurate biophysical information.

关 键 词：Single-particle tracking Photoactivatedlocalization microscopy - Single-moleculefluorescence imaging TGF-β receptor II Membranediffusion 

分 类 号：R3411[医药卫生—基础医学]