口岸蝇类携带致病菌及寄生虫卵的检测分析  

Detection of fly-borne pathogenic bacteria and parasite eggs collected from ports

作　　者：曹晓梅[1] 宋锋林[2] 姚李四[1] 张晓龙[1] 

机构地区：[1]中国检验检疫科学研究院,北京100176 [2]辽宁出入境检验检疫局,辽宁大连116001

出　　处：《中华卫生杀虫药械》2016年第2期148-152,共5页Chinese Journal of Hygienic Insecticides and Equipments

基　　金：国家质检总局科技计划项目(编号:2010IK215);国家科技支撑计划项目(编号:2012BAK11B05-02);国家科技重大专项(编号:2012ZX10004219-005)

摘　　要：目的了解口岸蝇类携带寄生虫卵与病原菌情况。方法将蝇体匀浆后进行细菌分离纯培养,采用梅里埃公司的全自动微生物鉴定系统,进行细菌鉴定;用离心沉淀法及饱和盐水浮聚法检测寄生虫卵。结果共鉴定出蝇类14属21种,分离出30种致病菌及条件致病菌,从输入性大头金蝇体内检测到肝吸虫卵和蛔虫卵。结论本研究为口岸蝇媒流行病学监测提供了技术支持。Objective To know the situation of fly-borne pathogenic bacteria and parasite eggs collected from ports.Methods Flies were homogenized for bacterial isolation and pure culture,and bacteria were identified with Merieux automated microbial identification system. Parasite eggs were detected by centrifugal sedimentation and saturated saline flotation. Results 14 family members including 21 species of fly were identified,and 30 species of pathogenic bacteria and condition pathogenic bacteria were isolated and identified. Liver fluke eggs and Ascaris eggs were detected from inputting Chrysomyia megacephala. Conclusion It provides a technical support for epidemiological surveillance.

关 键 词：口岸 蝇类 致病菌 寄生虫 检测 

分 类 号：R384.2[医药卫生—医学寄生虫学]