人尿道黏膜上皮细胞系与脱细胞羊膜基质复合培养的实验研究  

Research of composite culture of human urinary tract epithelial cells with human coacellular amniotic membrane matrix

作　　者：范巨峰[1] 芮宏亮[2] 宋森[3] 张岩崑[1] 范微微[1] 吕伟[1] 周璐[1] 李晶[1] 黄文罡[1] 侯莹[1] 

机构地区：[1]首都医科大学附属北京朝阳医院整形外科,北京100020 [2]卫生部中日友好临床研究所中心实验室 [3]清华大学医学院生物医学系

出　　处：《中国美容整形外科杂志》2016年第4期246-249,共4页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：北京市科技新星(2005B04);北京市卫生系统高层次人才(2011-3-019);北京市卫生局"十百千"人才计划

摘　　要：目的探讨人尿道黏膜上皮细胞系与脱细胞羊膜基质复合培养的效果。方法将人尿道黏膜上皮细胞系接种于脱细胞羊膜基质上,待细胞在脱细胞羊膜基质上生长至铺满表面,用活细胞示踪剂标记细胞,然后将脱细胞羊膜基质固定、封片,倒置相差和荧光显微镜下进行观察。结果人尿道黏膜上皮细胞系细胞在脱细胞羊膜基质上生长,细胞呈单层生长,活细胞示踪剂显示细胞生长良好,并具有活细胞功能。结论人尿道黏膜上皮细胞系是良好的实验室组织工程化尿道种子细胞,脱细胞羊膜基质是良好的实验室组织工程化尿道支架材料,适用于实验室组织工程化尿道复合过程研究。Objective To discuss the effect of composite culture of normal human urinary tract epithelial cells （SV-HUC-1） with human acellular amniotic membrane matrix （HAAM）. Methods SV-HUC-1 was inoculated on human acellular amniotie membrane matrix and the cells were tracked with viable cell tracer when growing on the surface of the amniotic membrane matrix. Then, the cells were observed with inverted phase contrast microscope and fluorescence microscope. Results SV-HUC-1 grew well on HAAM with normal monolayer morphology, which was proven by viable cell tracer. Conclusion SV-HUC-1 is a desirable seed cell for urinary tract tissue engineering and grows well on HAAM.

关 键 词：组织工程 人尿道黏膜上皮细胞系 脱细胞羊膜基质 复合培养 

分 类 号：R318.08[医药卫生—生物医学工程]