大气压低温氮等离子体对大鼠光老化皮肤的治疗研究  

Evaluation of Therapeutic Effect of Atmospheric Low Temperature Nitrogen Plasma on Photoaging Rat Skin

作　　者：许凌晖[1] 王达成[2] 许天星[1] 李坤杰[1] 杨思泽[3] 郭燕妮[1] 

机构地区：[1]福建医科大学附属第二医院皮肤科,泉州市362000 [2]华侨大学工学院 [3]厦门大学物理与机电学院.福建省等离子体与磁共振重点实验室

出　　处：《中国激光医学杂志》2016年第1期15-19,57-58,共7页Chinese Journal of Laser Medicine & Surgery

基　　金：福建省教育厅科技项目(JB11049);泉州市科技计划项目(2012Z55重点项目)

摘　　要：目的评估大气压低温氮等离子体治疗大鼠光老化皮肤的疗效。方法采用健康雌性SD大鼠40只,随机分为2个大组:正常对照组(A组)和模型组(B组),其中模型组又分为3个小组,分别为等离子体低剂量治疗组(B1组)、等离子体高剂量治疗组(B2组)和自然恢复组(B3组)。共计4组,每组10只。除正常对照组(A组)外,B组均采用UVA+UVB紫外线灯同时照射,每日2 h,连续照射60 d,制备大鼠皮肤光老化模型。SD大鼠光老化制模成功后,给予自制大气压低温氮等离子体治疗。B1组采用0.1 W/mm^2等离子体低剂量治疗,连续治疗4次,每次间隔时间2周;B2组采用0.3 W/mm^2等离子体高剂量治疗,连续治疗2次,每次间隔时间4周;B3组无等离子体治疗,自然恢复。每次治疗后观察各组大鼠皮肤即刻反应及恢复情况,分别于治疗前和治疗后1个月活检取材,进行常规HE染色和Masson三色染色。观察治疗前后大鼠皮肤的大体变化和组织病理学变化,并用Image-Pro-Plus 6.0病理图文分析系统测定大鼠皮肤胶原蛋白含量变化。结果采用等离子体治疗组大鼠皮肤光老化大体变化和病理改变明显改善。B1组和B2组与B3组比较胶原含量明显增加,差异具有显著意义(P<0.05)。B1组和B2组两组组间比较,疗效无明显差异。结论等离子体对皮肤光老化有明显的治疗作用,具有术后恢复快、不良反应低等优点。Objective To evaluate the effect of atmospheric low temperature nitrogen plasma regeneration technique in treating photoagingrats.Methods An experimental study was performed on 40 female SD rats.All rats were randomly assigned to two primary groups：normal control group A and model group B.The model group B were further divided into 3 secondary sub-groups：low-energy treatment group B1,high-energy treatment group B2 and model control group B3.As a result,the 40 SD rates were eventually randomized 1：1：1：1 to the 4 groups（A,B1,B2 and B3） with 10 SD rats in each group.Except those inthe group A,the SD ratswere irradiated with UVA plus UVB for 2 hours per day to establish a skin photoagingmodel.After 60 days,the skin photoaging model was established.The atmospheric low temperature nitrogen plasmadevice was designed and set up.The rats in group Bl were given 4 treatments in total,once every 2 weeks with the energy set at 0.1 W/mm2.In the meantime,The rats in group B2 underwent 2 treatments,once every 4 weeks with the energy set at 0.3 W/mm2.After each treatment,the degree of downtimeand erythema were recorded.Full-thickness skin biopsy specimens were obtained from each group before the treatment and 30 days following the last treatment respectively.Clinical characteristicswere observed and histological analysis was performed usingroutine Hematoxilin-Eosin staining and Trichrome-Masson staining.The content of dermal collagen wasmeasured with Image Pro-Plus 6.0 software.Results Clinical and histological observation showed that both plasma treatment groups（B1 and B2） were significantly improved,and the collagen increasedremarkably compared to the model control group（B3）,（P〈0.05）.There was no difference between low-energy treatment group（B1） and high-energy treatment group（B2）.Conclusions The atmospheric low temperature plasma regeneration technique is highly effective in treating photoaging skin with minimal down-time and few side effects.

关 键 词：光老化 动物模型 等离子体 治疗 胶原纤维 

分 类 号：R454.2[医药卫生—治疗学]