LC-MS/MS法测定安喘舒丸中腺苷和黄芪甲苷在人血浆中的蛋白结合率  

Determination of protein-binding rate of adenosine and astragaloside Ⅳ in Anchuanshu pills in human plasma by LC-MS/MS

作　　者：许多[1] 刘梅[1] 杨波[1] 尹琼玉 陆瑜[1] 

机构地区：[1]解放军第四五四医院药学部,南京210002

出　　处：《中南药学》2016年第3期282-285,共4页Central South Pharmacy

摘　　要：目的研究安喘舒丸中腺苷和黄芪甲苷在人血浆中的蛋白结合率。方法采用LC-MS/MS法测定腺苷和黄芪甲苷与人血浆蛋白结合率,LC-MS/MS法测定透析内、外液中2种成分质量浓度,并计算血浆蛋白结合率。结果腺苷和黄芪甲苷的线性关系、精密度与准确度、提取回收率和稳定性均符合方法学要求。安喘舒丸低、中、高(30、60、120 mg·L-1)3个浓度中,腺苷的人血浆蛋白结合率分别为(34.78±1.38)%、(35.43±1.04)%、(35.29±1.63)%;黄芪甲苷为(86.69±1.72)%、(86.14±2.37)%、(85.42±2.55)%。结论安喘舒丸中腺苷与人血浆具有低强度蛋白结合率,黄芪甲苷与人血浆具有高强度蛋白结合率。Objective To determine the protein binding rate of adenosine and astragaloside IV in Anchuanshu pills in human plasma. Methods LC-MS/MS method was used to determine the binding rates in vitro, and the determination of adenosine and astragaloside IV both in inner and outer dialysis. Results The linearity, preci- sion and accuracy, extraction recovery and stability of adenosine and astragaloside IV all met the methodology requirements. Within the low, middle and high concentration （30, 60, and 120 mg·L - 1） of Anchuanshu pills, the protein binding rate of adenosine was （34.78±1.38）%, （35.43±1.04）% and （35.29±1.63）%; while the pro- tein binding rate of astragaloside IV was （86.69±1.72）%, （86.14±2.37）% and （85.42±2.55）%. Conclusion In Anchuanshu pill, adenosine has low protein binding rate in human plasma, while astragaloside IV has high binding rate.

关 键 词：腺苷 黄芪甲苷 安喘舒丸 血浆蛋白结合率 平衡透析法 LC-MS/MS法 

分 类 号：R285[医药卫生—中药学]