长枝木霉Trichoderma longibrachiatum T6水分散粒剂的研制  

Research and development of water dispersible granules of Trichoderma longibrachiatum T6

作　　者：景芳[1] 徐秉良[1] 梁巧兰[1] 张树武[1] 刘佳[1] 

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,兰州730070

出　　处：《农药学学报》2016年第2期241-248,共8页Chinese Journal of Pesticide Science

基　　金：甘肃省农牧厅生物技术专项(GNSW-2013-19);甘肃省农牧厅生物技术研究与开发应用项目(GNSW-2009-04);甘肃省教育厅项目(042-03);甘肃省高校基本科研业务费项目(甘财教[2012]129号)

摘　　要：以土壤中分离得到的一株拮抗菌——长枝木霉Trichoderma longibrachiatum T6为研究对象,通过优化培养基配方及助剂类型的筛选,成功研制了长枝木霉T.longibrachiatum T6水分散粒剂,并测定了其贮存稳定性。结果表明：制备长枝木霉T6水分散粒剂的最佳配方（质量分数）为：羧甲基纤维素（紫外保护剂）0.5%,碳酸钙（稳定剂）4%,聚乙烯基吡咯烷酮（PVP,黏结剂）5%,可溶性淀粉（崩解剂）5%,凹凸棒土（载体）5%及十二烷基硫酸钠（润湿剂）5%。由此配方制备的水分散粒剂中孢子含量为5.6×10^8 cfu/g,悬浮率53%,p H值6.0,水分含量3.5%,湿润时间52 s,且在冷藏温度为（4±2）℃条件下的稳定性显著高于在室温（25℃）下的。各项检测结果均符合国家标准。The water dispersible granules of Trichoderma longibrachiatum T6 was developed. This antagonistic bacteria was isolated from field soil. The T. longibrachiatum T6 water dispersible granules were developed by optimizing media formulations and screening the aid types. And the storage stability was measured. The results indicated that the best formulation（mass fraction） was hydroxymethyl cellulose（UV protective agents） 0.5%, calcium carbonate（stabilizing agents） 4%, polyvinylpyrrolidone（PVP, binders） 5%, soluble starch（disintegrating agents） 5%, attapulgite（carriers） 5%, sodium lauryl sulfate（witting agents）5%. The quality detection showed that the spores production of T.longibrachiatum T6 water dispersible granules was 5.6 × 10^8 cfu/g, the suspension rate of the production was 53%, p H was 6.0, the moisture amount was 3.5%, the wetting time was 52 s, the stability in low temperature of（4 ± 2） ℃ was significantly higher than that at room temperature 25 ℃,which came up to the formulation standards.

关 键 词：木霉 水分散粒剂 培养基质 产孢量 稳定性 

分 类 号：TQ450.6[化学工程—农药化工] S476[农业科学—农业昆虫与害虫防治]