六种杀菌剂对烟草赤星病菌菌丝生长和分生孢子萌发的抑制作用  

Inhibitory activities of six fungicides against mycelial growth and conidial germination of Alternaria alternata

作　　者：黄艳飞[1] 汪汉成 陈庆元 王进[3] 张长青[1] 鲁红学[1] 

机构地区：[1]长江大学农学院,湖北荆州434025 [2]贵州省烟草科学研究院,贵阳550081 [3]长江大学生命科学学院,湖北荆州434025

出　　处：《农药学学报》2016年第2期263-267,共5页Chinese Journal of Pesticide Science

基　　金：国家自然科学基金项目(31360448;31501679);中国烟草总公司贵州省公司科技项目(201305;201336;201436)

摘　　要：采用菌丝生长速率法和孢子萌发法测定了烟草赤星病菌Alternaria alternata对6种杀菌剂(氟硅唑、异菌脲、菌核净、嘧菌酯、醚菌酯及多菌灵)的敏感性。结果表明:6种杀菌剂对烟草赤星病菌菌丝生长和分生孢子萌发均表现出了不同程度的抑制活性。其中,对菌丝生长抑制活性最强的是氟硅唑,EC50平均值为(0.34±0.11)mg/L,其次为菌核净[(1.13±0.68)mg/L]和异菌脲[(2.08±0.24)mg/L];对分生孢子萌发抑制活性最强的是嘧菌酯协同100 mg/L水杨肟酸,EC50平均值为(0.28±0.09)mg/L,其次分别为单独处理的嘧菌酯[(5.79±2.76)mg/L]、醚菌酯[(11.93±3.93)mg/L]、菌核净[(19.95±15.94)mg/L]和异菌脲[(23.33±11.33)mg/L]。多菌灵对烟草赤星病菌菌丝生长和分生孢子萌发的抑制活性均最差,不宜用于烟草赤星病的防治,而异菌脲、氟硅唑、嘧菌酯和醚菌酯均有必要进一步针对烟草赤星病进行田间防效试验。Activities of six different fungicides（flusilazole, iprodione, dimethachlon, azoxystrobin,kresoxim-methyl, carbendazim） to Alternaria alternata were determined by mycelial growth rate method and conidial germination assay. The results showed that the six fungicides showed different inhibitory activities against A. alternata on mycelial growth and conidial germination. The most effective fungicide against the mycelial growth of A. alternata was flusilazole, with average EC50 value of（0.34 ± 0.11） mg/L, followed by dimethachlon [（1.13 ± 0.68） mg/L] and iprodione [（2.08 ± 0.24）mg/L]. The most effective fungicide against theconidial germination of A. alternata was azoxystrobin in combination with 100 mg/L SHAM, with average EC50 value of（0.28 ± 0.09） mg/L, followed by azoxystrobin [（5.79 ± 2.76） mg/L], kresoxim-methyl [（11.93 ± 3.93） mg/L], dimethachlon [（19.95 ±15.94） mg/L] and iprodione [（23.33 ± 11.33） mg/L] when used alone. Carbendazim showed the lowest inhibition on both mycelial growth and conidial germination of A. alternata, suggesting it should not be used for controlling tobacco brown spot. Further study is needed to investigate the field control efficacy of iprodione, flusilazole, azoxystrobin and kresoxim-methyl against tobacco brown spot.

关 键 词：烟草赤星病 杀菌剂 菌丝生长 孢子萌发 抑制活性 

分 类 号：S481.1[农业科学—农药学] S482.2[农业科学—植物保护]