外源NO对低温胁迫下苦瓜幼苗生长和几个生理指标的影响  

Effects of Exogenous NO on Plant Growth and Physiological Characteristics in Momordica charantia L. Seedlings Under Low Temperature Stress

作　　者：杜卓涛[1] 商桑[1] 朱白婢[2] 田丽波[1] 杨衍[3] 

机构地区：[1]海南大学园艺园林学院热带作物种质资源保护与开发利用教育部重点实验室,海南海口570228 [2]海南省农业科学院蔬菜研究所,海南海口570228 [3]中国热带农业科学院作物品种资源研究所,海南儋州571737

出　　处：《热带作物学报》2016年第3期482-487,共6页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金(No.311029);国家自然科学基金(No.31460517);海南省蔬菜生物学重点实验室开放课题(No.SCKF201502);国家科技支撑计划项目子课题(No.2014BAD05B04);海南大学中西部计划学科建设项目(No.ZXBJH-XK008)

摘　　要：为探讨外源NO提高苦瓜抗冷性的内在机制,以碧绿苦瓜幼苗为试材,对其施加不同浓度(0.00、0.10、0.50、1.00、1.50、2.00 mmol/L)的外源NO供体硝普钠(SNP)并进行低温(8℃)处理,研究SNP对低温胁迫下苦瓜幼苗的生长及生理指标的影响。结果显示,SNP处理提高了叶片相对含水量、可溶性蛋白含量及总叶绿素、叶绿素a的含量,与对照相比,株高、茎粗、叶面积和根体积均有增加。低浓度的SNP处理的苦瓜幼苗抗冷效果好于高浓度的,尤其以0.50 mmol/L SNP处理效果最佳。研究结果表明,适宜浓度的外源NO能够提高幼苗的持水能力,促进渗透调节物质的合成,缓解低温导致的叶绿素降解,提高低温胁迫下植株的生物量,从而增强植株的抗冷性。In this experiment, a Momordica charantia L. variety Bilvwas used, and different concentrations of sodium nitroprusside（SNP）（0.00, 0.10, 0.50, 1.00, 1.50 and 2.00 mmol/L） was employed as the exogenous nitric oxide（NO） donor to process M. charantia L. seedlings under 8℃ low-temperature stress to study the internal mechanism of exogenous NO improving the cold resistance of M. charantia L.. The results showed that the treatment of SNP increased relative water content, total chlorophyll, chlorophyll a, chlorophyll b and soluble protein content of the leaves; Plant height, stem diameter, leaf area and root volume increased than the control, and the cold resistance of the seedlings in the field was improved. It was also found that the cold resistance effect of the treatment with low concentration of SNP was bitter than that of the concentration treatment, especially the treatment with0.50 mmol/L SNP was the best. It was indicated that the exogenous NO with proper concentration could promote the plant growth, soluble protein content, relative water content and chlorophyll content to enhance the the seedling adaptability for low temperature stress.

关 键 词：苦瓜 外源NO 低温胁迫 抗冷性 

分 类 号：S642.5[农业科学—蔬菜学]