SgP5CS基因克隆和生物信息学分析  

Molecular cloning and bioinformatics analysis of SgP5CS gene in Suaeda glauca

作　　者：金杭霞[1] 董德坤[1] 王伟 杨清华[1] 朱丹华[1] 

机构地区：[1]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021 [2]萧山区种子管理站,浙江杭州311201

出　　处：《浙江农业学报》2016年第3期395-399,共5页Acta Agriculturae Zhejiangensis

基　　金：中国博士后科学基金面上项目(2014M551772);国家转基因生物新品种培育重大专项(2014ZX0800403B)

摘　　要：利用同源克隆的方法,获得了碱蓬P5CS基因全长ORF,命名为SgP5CS。SgP5CS ORF全长2 151 bp,生物信息学软件预测其编码716个氨基酸组成的多肽,相对分子量为77 431.8 u,等电点为5.71,为稳定的亲水性蛋白,无跨膜结构域。Clustal X多重序列比对发现SgP5CS编码的氨基酸序列与盐角草P5CS相似性为93%,与盐角草P5CS的亲缘关系最近,其次是甜菜。脯氨酸是生物体内重要的渗透调节剂,而Δ1-吡咯啉-5-羧酸合成酶则是植物合成脯氨酸的关键酶之一,SgP5CS的克隆将为进一步的功能分析鉴定基础。A new gene,named as SgP5CS,was cloned by homologous cloning method from Suaeda glauca. Bioinformatics analysis showed that the open reading frame of SgP5CS gene was 2 151 bp,encoding 716 amino acids. The isoelectric point and the relative molecular weight of coded protein were 5. 71 and 77 431. 8 u,respectively. Hydrophobic analysis indicated that SgP5CS was a hydrophilic protein and had no transmembrane domain. Nucleotide sequence Blast by Clustal X indicated that the coded protein of SgP5CS shared an identity of 93% with P5CS of Salicornia europaea. Δ1-pyrroline-5-carboxylate synthetase（ P5CS） is a key enzyme in the synthesis of proline which is one of the important osmotic regulators in plants. Our research laid a foundation to further study the P5CS function in Suaeda glauca.

关 键 词：碱蓬 P5CS 脯氨酸 

分 类 号：Q943.2[生物学—植物学]