HPLC法测定乌蕨中酚酸类成分含量研究  

Determination of two Phenolic Compounds in Stenolomachusanum(Linn.)Ching by HPLC

作　　者：李小芳[1,2] 罗国安[2] 朱婉婷[1,3] 

机构地区：[1]江西中医药大学,江西南昌330004 [2]清华大学,北京100084 [3]广东药学院中药学院,广东广州510006

出　　处：《亚太传统医药》2016年第8期36-39,共4页Asia-Pacific Traditional Medicine

摘　　要：目的：采用RP-HPLC法测定不同产地乌蕨中原儿茶酸、原儿茶醛2个酚酸化合物的含量。方法：色谱柱为依利特ODS C18（4.6mm×250mm,5μm）,以甲醇-0.1%磷酸水溶液（25∶75）为流动相,流速：1.0mL/min,检测波长：260nm,柱温：25℃。结果：原儿茶酸在0.14~2.8μg范围内线性关系良好（r=0.999 8）,平均回收率为100.19%（RSD=0.12%）;原儿茶醛在0.06~1.2μg范围内线性关系良好（r=0.999 9）,平均回收率为98.65%（RSD=1.71%）。结论：该方法简便、快速、准确可靠,可为乌蕨的质量控制提供依据。Objective：To establish an RP-HPLC determination of Protocatechuic acid and Protocatechuic aldehyde in Stenolomachusanum（Linn.）Ching from different sources.Methods：The separation was performed on a Hypersil ODS C_（18）（4.6mm×250mm,5μm）column with the gradient elution methanol-0.1% phosphoric acid（25∶75）;The flow rate was 1.0mL/min;The detection wavelength was 260nm;The column temperature was at 25℃.Results：Linear ranges of Protocatechuic acid and Protocatechuic aldehyde were 0.14~2.8μg（r=0.999 8）,0.06~1.2μg（r=0.999 9）.The average recoverys were 100.19%（RSD=0.12%）and98.65%（RSD=1.71%）（n=6）,respectively.Conclusion：The method is simple,rapid,accurate and which may also be applied as a standard for the quality control of Stenolomachusanum（Linn.）Ching.

关 键 词：乌蕨 原儿茶酸 原儿茶醛 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]