银鲳ERα基因片段的克隆及卵黄发生期间饲料n-3LC-PUFA对其组织表达的影响  

Molecular cloning of ERα from silver pomfret Pampus argenteus and the response of its expression to dietary n-3 LC-PUFA levels during vitellogenesis

作　　者：彭士明[1] 李云莉[1,2] 高权新[1] 施兆鸿[1] 张晨捷[1] 王建钢[1] 

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《海洋渔业》2016年第2期157-165,共9页Marine Fisheries

基　　金：国家自然基金项目(31202009);上海市科技兴农重点攻关项目(沪农科攻字2013-2-1);中央级公益性科研院所基本科研业务费(东2014Z02-2)

摘　　要：本研究克隆获得银鲳（Pampus argenteus）雌激素受体α（estrogen receptorα,ERα）基因的部分c DNA序列,长度196 bp,编码65个氨基酸。经BLAST比对,与其它鱼类ERα基因序列的一致性在89%~93%之间,证明实验所得序列为银鲳ERα基因的部分片段。以1年龄雌性银鲳为实验对象,配制了4组等氮、等能及等脂的实验饲料,分别以100%鱼油（FO组）、70%鱼油和30%大豆油（FSO组）、30%鱼油和70%大豆油（SFO组）、100%大豆油（SO组）为脂肪源,研究银鲳在卵黄发生期间组织中ERα基因表达量的变化以及饲料n-3LC-PUFA对其组织表达的影响,实验周期185 d。结果表明,在卵黄发生中期与后期,肝脏与卵巢中ERα基因表达量均显著高于卵黄发生前期（P〈0.05）;卵黄发生后期肝脏组织中ERα基因表达量虽较卵黄发生中期呈升高趋势,但除了SO组之外,两者之间无显著性差异（P〉0.05）;而卵巢组织中ERα基因表达量在整个卵黄发生期间均呈现显著升高趋势（P〈0.05）。饲料n-3LC-PUFA对肝脏与卵巢ERα基因表达量的影响在卵黄发生前期均未表现出明显的组间差异,但在卵黄发生中期与后期,饲料中较高的n-3LC-PUFA含量显著提高了组织中ERα基因表达量。在卵黄发生中期与后期,FO与FSO组肝脏与卵巢组织中ERα基因表达量均分别显著高于SO组（P〈0.05）。双因素方差分析结果表明,饲料n-3LC-PUFA与卵黄发生时期对银鲳组织中ERα基因表达量均具有显著性影响,且两者对组织中ERα基因表达量存在显著性的交互作用。It has been reported that n-3 long-chain polyunsaturated fatty acids（ n-3 LC-PUFAs） such as eicosapentaenoic acid（ EPA,20： 5n-3） and docosahexaenoic acid（ DHA,22∶ 6n-3） play critical roles in cell permeability and enzyme activity. Marine teleosts can not synthesize LC-PUFAs from 18-carbon precursors due to a deficiency in Δ5-desaturase. Therefore,it is imperative that teleost diets contain LC-PUFAs. The importance of LC-PUFAs, especially n-3 LC-PUFAs, in fish broodstock nutrition has been extensively studied. Dietary n-3 LC-PUFAs affect spawning performance,egg and larval quality,and egg fatty acid composition of broodstock. In humans,fatty acids,especially PUFAs,are cell signaling molecules,structural compounds and energy sources. PUFAs are crucial for mediating immunological,metabolic,and endocrine signals within the uterine-fetal-placental unit. PUFAs are precursors of cell signaling molecules（ e. g.,eicosanoids）,and there is evidence that they regulate membrane signal transduction pathways and steroid hormone action,by modulating the binding of estrogen,progesterone,and glucocorticoids to their intracellular receptors. Therefore,PUFAs act as endogenous modulators of key enzymes involved in steroid metabolism.While most studies have focused on the effects of diet on body composition,growth performance,egg quality and larval survival rate,few studies have investigated the effects of nutrients on hormone synthesis during gonadogenesis. Pituitary gonadotropin hormone（ Gt H）,follicle-stimulating hormone（ FSH）,and luteinizing hormone（ LH） are involved in gametogenesis and sexual maturation in teleosts. In response to Gt H,cholesterol is converted into testosterone（ T） and 17β-estradiol（ E2）. Sex steroid hormones play important roles in vertebrate reproduction. In several teleost species,E2 and T,which are produced in gonadal tissues under the control of Gt H,are essential in gametogenesis. Estrogen receptors（ ER） are members of steroid receptor family of ligand-activate

关 键 词：银鲳 雌激素受体Α 卵黄发生 n-3LC-PUFA 基因表达 

分 类 号：S968.1[农业科学—水产养殖]