盐度骤降对云纹石斑鱼肝脏代谢酶活力的影响  

Impact of abrupt salinity decrease on metabolic enzymes in the liver of Epinehelus moara

作　　者：施兆鸿[1,2] 廖雅丽[1,2] 王孝杉[2] 高权新[2] 彭士明[2] 张晨捷[2] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院东海水产研究所,上海200090

出　　处：《海洋渔业》2016年第2期174-181,共8页Marine Fisheries

基　　金：中央级公益性科研院所基本科研业务费项目(东2015M04)

摘　　要：为探讨云纹石斑鱼(Epinephelus moara)肝脏代谢酶活力对盐度骤降的响应,设置27(对照组)、21、15、9共4个盐度梯度对云纹石斑鱼进行盐度骤降胁迫实验。分别在0、1、2、3、7 d时取样,测定其肝脏中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、谷丙转氨酶(GPT)、谷草转氨酶(GOT)以及乳酸脱氢酶(LDH)活力。结果显示,ACP活力盐度21组先波动变化后恢复正常水平,盐度15组先下降后升高,随后第7天下降恢复正常水平;AKP活力盐度21组和盐度15组先呈下降趋势,第3天大幅度升高;GPT与LDH活力盐度21组和盐度15组基本呈现先下降再上升并波动的变化趋势;GOT活力变化趋势盐度21组先升后降,盐度15组先降后波动。研究表明:在盐度27骤降至21或15,均会使云纹石斑鱼产生应激反应,磷酸酶、转氨酶与乳酸脱氢酶活力初期均会受到抑制,随时间延长可以得到恢复;而当盐度从27骤降至9,3 d时实验鱼全部死亡。This experiment was conducted to investigate the response of metabolic enzymes in the liver of Epinehelus moara to abrupt salinity decrease. Four salinity groups（ 27,21,15,9） were designed. The average body length of Epinehelus moara was（ 10. 43 ± 1. 08） cm and the average body weight was（ 16. 73 ±4. 47） g. The fish were reared temporarily in the water of（ 27 ± 1. 0） ℃ temperature and 27 salinity when reaching Shanghai Fisheries Research Institute of Qidong breeding base. The study was conducted in a circular glass cylinder barrel with 1 m in diameter and 0. 8 m in depth. The temporary rearing seawater at salinity 27 was the control group,and the seawater with lower salinities（ salinity 21,salinity 15,salinity 9） used tap water after aeration mixed with natural seawater after purification treatment,and the experimental water could be used after stabling for 48 hours. Every salinity gradient had 3 parallels and 12 buckets. Fish livers in each group were sampled on day 0,1,2,3,and 7 to measure the activities of acid phosphatase（ ACP）,alkaline phosphatase（ AKP）,glutamic-pyruvic transaminase（ GPT）,glutamic-oxalacetic transaminase（ GOT） and lactate dehydrogenase（ LDH）. Results showed that the activity of ACP in salinity 21 group first changed wavely,then recovered to the nomal level. ACP activity in salinity 15 group decreased first and then increased,finally decreased to the normal level on day 7; AKP activity in salinity 21 and 15 groups decreased on day 1 and 2,and then increased significantly on day 3; GPT and LDH activities in salinity 21 and 15 groups first decreased and then increased wavely; GOP activities in 21 salinity group increased first and then decreased,activities in salinity 15 group decreased first and then changed wavely. The results indicated that abrupt salinity decrease from 27 to 21 or 15 could cause stress response of Epinehelus moara; the phosphatase,aminopherase and LDH activities were all inhibited first and recovered afterward; the abrupt salinit

关 键 词：云纹石斑鱼 骤降低盐胁迫 肝脏代谢酶 

分 类 号：S917[农业科学—水产科学]