濒危植物珠子参愈伤组织诱导因素分析  

Different Factors on Callus Induction of Panax japonicus var.major

作　　者：李利霞[1] 李婷婷[1] 朱虹[1] 孙长生[1] 刘雪兰[1] 赵厚涛[1] 

机构地区：[1]贵阳药用植物园,贵州贵阳550002

出　　处：《北方园艺》2016年第7期85-88,共4页Northern Horticulture

基　　金：贵州省中药现代化科技产业研究开发专项资助项目(ZY字[2012]3005号);贵阳市科技计划资助项目(2012HK-209-37)

摘　　要：以珠子参节、节间和越冬芽萌发后的叶、根为试材,研究了不同消毒方法、外植体、植物生长调节剂及培养条件等对珠子参愈伤组织诱导的影响,以期获得愈伤组织诱导的最佳方法。结果表明:最佳消毒方法是75%酒精消毒15s后,越冬芽、节和节间分别加0.2%氯化汞消毒5、15、15min;节间为愈伤组织诱导最适宜外植体;2,4-D 2.0mg/L+KT 0.1mg/L对节间诱导愈伤组织效果最好,诱导率为96.67%;以MS为基础培养基添加浓度为5g/L的琼脂较适宜愈伤组织的诱导;温度25℃、光周期24h/d、光照强度6 200lx的条件有利于愈伤组织的诱导。Taking knobs,internode and leaves,roots of winter buds of Panax japonicus var.majoras experiment material,the effects of different disinfection method,explants,plant growth regulator and culture conditions on callus induction were studied to find the best method of callus induction.The results showed that the better disinfection treatment was 15 seconds with 75% C_2H_5 OH,then 5minutes with 0.2% HgCl_2 for winter bud,but 15 minutes for knobs and internode.The internode was the best explant for callus induction.The induction rate 96.67% was the highest with 2,4-D2.0mg/L＋KT 0.1mg/L for internode.The MS medium with agar 5g/L was suitable for induction of callus.Condition of the temperature 25℃,the photoperiod of 24h/d and the light intensity of 6 200 lx was beneficial for callus induction.

关 键 词：珠子参 组织培养 愈伤组织 

分 类 号：S567.53[农业科学—中草药栽培]