铜绿丽金龟对寄主植物挥发物的触角电生理及行为反应  

EAG and behavioral responses of copper-green chafer Anomala corpulenta Motschulsky(Coleoptera:Rutelidae)

作　　者：鞠倩[1] 郭晓强[1] 李晓[1] 蒋相国[2] 倪婉莉 曲明静[1] 

机构地区：[1]山东省花生研究所,青岛266100 [2]湖北省襄阳市农业科学院,襄阳441057 [3]安徽省农业科学院,合肥230031

出　　处：《植物保护学报》2016年第2期281-287,共7页Journal of Plant Protection

基　　金：国家现代农业(花生)产业技术体系(CARS-14);科技部农业科技成果转化资金项目(2013GB2C620547);山东省农业科学院青年科研基金(2014QNM26)

摘　　要：为深入了解铜绿丽金龟的取食行为,探索开发安全高效的植物源引诱剂,应用昆虫触角电位反应仪和Y型嗅觉仪测试其对不同寄主植物挥发物的趋向行为差异,并根据室内行为结果配制诱剂进行大田试验。结果表明:铜绿丽金龟雄虫对水杨酸甲酯和(1,1’-联环戊基)-2-酮的触角电位反应(electroantennography,EAG)值显著高于其它试剂;石竹烯和水杨酸甲酯能引起雌虫触角较强的电位反应;乙酸顺式-3-己烯酯和石竹烯分别对雄虫和雌虫有较高的嗅觉选择反应率,分别达0.95和0.94。综合EAG和嗅觉试验结果,选择对雌、雄虫均有较好引诱效果的反式-2-己烯醛、乙酸顺式-3-己烯酯、石竹烯、(1,1’-联环戊基)-2-酮和水杨酸甲酯进行田间试验,最终筛选到铜绿丽金龟雄虫的最适引诱剂为每诱芯360 mg乙酸顺式-3-己烯酯,雌虫的最适引诱剂为每诱芯360 mg石竹烯,日诱虫量分别可达33.00±1.53头和29.33±1.45头。In order to understand the feeding behavior of Anomala corpulenta（Coleoptera）,explore safe and efficient plant-derived attractant,the behavioral responses of A.corpulenta to odors from leaves of different host plants were tested by using electroantennography（EAG）,Y-shaped olfactometer and field trapping test.The results showed that the EAG value of A.corpulenta males to 2-carbomethoxyphenol and1,1＇-bi（cyclopentyl）-2-one were significantly higher than to the other chemicals tested;the EAG value of females to（-）-E-caryophyllene and 2-carbomethoxyphenol were significantly higher than to other reagents.According to the Y-shaped bioassays,（Z）-3-hexenyl acetate and（-）-E-caryophyllene were found to be more attractive to males and females of A.corpulenta,respectively.The results of field trapping test with（E）-2-hexenal,（Z）-3-hexenyl acetate,（-）-E-caryophyllene,1,1＇-bi（cyclopentyl）-2-one and 2-carbomethoxyphenol showed that cis-3-hexenyl acetate（360 mg /lure） attracted the highest number（33 ± 1.53） of males,while（-）-E-caryophyllene（360 mg /lure） attracted the highest number（29.33 ± 1.45） of females.

关 键 词：铜绿丽金龟 寄主植物挥发物 触角电位 嗅觉反应 田间诱捕试验 

分 类 号：S433[农业科学—农业昆虫与害虫防治]