1株花柳珊瑚来源真菌Aspergillus candidus次级代谢产物及其生物活性研究  

Study on secondary metabolites from the fungus Aspergillus candidus derived from the gorgonian coral Anthogorgia ochracea and their biological activities

作　　者：马洁[1] 郑纪勇[2] 孙泽涛[1] 王长云[1] 邵长伦[1] 

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003 [2]海洋腐蚀与防护重点实验室,中国船舶重工集团公司第七二五研究所,山东青岛266061

出　　处：《中国海洋药物》2016年第2期18-22,共5页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(41176121,41322037);科技部973计划项目(2014CB643305)资助

摘　　要：目的从中国南海花柳珊瑚来源真菌Aspergillus candidus（RA16-10）中分离鉴定具有生物活性的代谢产物。方法运用硅胶柱层析、Sephadex LH-20凝胶柱层析和半制备HPLC等方法分离并分析化合物,运用核磁、质谱等现代波谱分析方法对化合物进行结构鉴定,并对化合物进行抗菌、卤虫致死、藤壶幼虫致死等活性评价。结果从A.candidus中分离鉴定了3个黄酮类化合物（1～3）和1个三联苯类化合物（4）,其中含有氯原子的黄酮类化合物1和3是自然界中极其罕见的化合物;化合物1和2显示出强的藤壶幼虫Balanua amphitrite致死活性,其LC_（50）值分别为1.11μg/mL和1.39μg/mL,这是首次报道该类化合物的藤壶幼虫致死活性。结论从中国南海花柳珊瑚来源真菌A.candidus（RA16-10）中筛选发现了2个具有显著的藤壶幼虫B.amphitrite致死活性的黄酮类化合物,这类化合物具有开发成为防污剂的潜力。Objective To isolate bioactive marine natural compounds from the fungus Aspergillus candidus（RA16-10）derived from the gorgonian coral Anthogorgia ochracea collected from the South China Sea.Methods The compounds isolated from the coral-derived fungus A.candidus（RA16-10）were separated and purified by Silica gel column chromatography,Sephadex LH-20 gel and HPLC and the structures of these isolated compounds were identified by modern spectroscopic analysis including NMR and MS methods.In addition,their biological activities including antimicrobial activity,brine shrimp lethality and barnacles lethality were evaluated.Results Three flavonoids（1～3）and one terphenyl compound（4）were isolated fromA.candidus Flavonoids with chlorine atoms were very unique from natural materials.Compounds 1 and 2 were evaluated the lethality of barnacle larvae（B.amphitrite）and both of them exhibited strong lethal activity with LC50 values of 1.11μg/mL and 1.39μg/mL,respectively.The antifouling activity of these two metabolites was detected for the first time.Conclusion Two flavonoids with potent antifouling activity isolated from the fungus Aspergillus candidus（RA16-10）derived from a gorgonian coral A.ochraceadisplayed good lethal activity against barnacles,which suggested that they were a promising class of antifouling agent.

关 键 词：花柳珊瑚 ASPERGILLUS candidus 藤壶幼虫致死活性 

分 类 号：R931[医药卫生—生药学]