新疆南疆核桃树腐烂病菌鉴定  

Identification of Canker Pathogen of Juglans in Southern Xinjiang

作　　者：郭开发[1] 王刚[1] 吴彩兰[1] 包亚洲 赵思峰[1] 

机构地区：[1]新疆绿洲农业病虫害治理与植保资源利用自治区高校重点实验室/石河子大学农学院,新疆石河子832003

出　　处：《新疆农业科学》2016年第3期496-501,共6页Xinjiang Agricultural Sciences

基　　金：新疆生产建设兵团青年科技创新资金专项"新疆主要林木腐烂病菌种类鉴定及其关键防治技术研究"(2014CB002)~~

摘　　要：【目的】明确新疆南疆核桃腐烂病菌种类。【方法】从南疆3个地方采集核桃腐烂病样10份,采用组织分离法分离获得10个分离物,根据科赫氏法则进行致病性测定,结合形态学观察和18 S rDNA-ITS、β-tubulin两个基因序列分析。【结果】经形态学特征鉴定,菌落形态、分生孢子器、分生孢子与已报道的金黄壳囊孢Cytospora chrysosperma一致;从18 S rDNA-ITS、β-tubulin两个基因序列分析可以得出,18 S r DNA-ITS序列分析结果与C.chrysosperma(登录号:KP114125.1)相似性为99%,β-tubulin序列分析结果与C.chrysosperma(登录号:KP117038.1)相似性达到98%。【结论】新疆南疆核桃树腐烂病菌为金黄壳囊孢C.chrysosperma。【Objective】To isolate and identify canker pathogen of Juglans in Xinjiang.【Method】A total of 10 canker samples in Juglans were collected and 10 isolates were obtained by tissue isolation,and pathogenicity was determinaed according to Koch＇s rule. These isolates were identified by means of the morphological features of culture,18 S r DNA- ITS and β- tubulin gene sequences analysis.【Result】Through colony morphology,pycnidium,conidia of the isolates was consistent with the reported Cytospora chrysosperma. ITS identification showed the sequence was up to 99% identical with C. chrysosperma（ KP114125. 1）,β- tubulin identification showed the sequence was up to 98% identical with C. chrysosperma（ KP117038. 1）.【Conclusion】These isolates from Xinjiang were identified as C. chrysosperma.

关 键 词：核桃 腐烂病 鉴定 金黄壳囊孢 

分 类 号：S436.64[农业科学—农业昆虫与害虫防治]