植物凝集素PHA-L对小鼠急性辐射防护作用  

Protective effects of the plant lectin PHA-L on acute radiation injuries in mice

作　　者：张广慧[1] 吴红英[1] 靳瑾[1] 周则卫[1] 刘培勋[1] 龙伟[1] 

机构地区：[1]中国医学科学院北京协和医学院放射医学研究所,天津300192

出　　处：《辐射研究与辐射工艺学报》2016年第2期12-17,共6页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金青年项目(81202153);教育部博士点新教师基金(20121106120042);天津市应用基础与前沿技术研究计划青年项目(14JCQNJC10900);中国医学科学院放射医学研究所发展基金(1523)资助~~

摘　　要：观察植物凝集素PHA-L对小鼠急性辐射损伤的保护作用及相关机制。采用动物30 d存活率实验,即小鼠全身辐照至吸收剂量为7.2 Gy后观察其存活情况;利用外周血和免疫学实验,小鼠全身照射至吸收剂量为7.2 Gy后测外周血白细胞数(White blood cell,WBC)、股骨有核细胞数(The number of nucleated cells in bone marrow,BMNC)、骨髓DNA含量以及各脏器指数;对小鼠进行9.0 Gy腹部照射,取小肠组织做病理切片观察。结果显示,照射给药组小鼠30 d存活率比单纯照射组均有提高,特别是高剂量组提高约60%;与单纯照射组相比,照射给药高剂量组的白细胞数、骨髓DNA含量和脾结节数均有提高,分别从0.74±0.16、1.02±0.17和9.80±6.46提高到1.18±0.40、1.22±0.17和18.10±6.87,数据均有统计学意义(p<0.05),并且PHA-L对脏器也有一定的保护作用;与单纯照射组相比,照射给药高剂量组小鼠肠道组织损伤恢复明显。提示植物凝集素PHA-L对急性辐射损伤具有保护作用,但其作用机制有待进一步研究。The aim is to observe the protective effects of plant lectin PHA-L against radiation injuries and the relative mechanism. 30 d survival experiment was used to observe the survival condition of mice 30 d after irradiation with 7.2 Gy ,Y-rays. Blood and immunological experiments of mice were carried out to observe WBC （White blood cell）, femoral nucleated cells, bone marrow DNA content and organs index after irradiation with 7.2 Gy y-rays. Intestine pathological section of mice irradiated on abdomen with 9.0 Gy Y-rays was prepared to observe the pathology of intestine. The results showed that compared with the irradiation group, the survival rate of mice treated with PHA-L increased, especially for the high dose group （60% lifted）; compared with the irradiation group, whiteblood cell count, bone marrow DNA content and spleen nodules of mice administered with high dose group increased from 0.74±0.16, 1.02±0.17 and 9.80±6.46 to 1.18±0.40, 1.22±0.17 and 18.10±6.87 respectively, which were statistically significant （p〈0.05）. Moreover, PHA-L also had some protective effect on the organ. In pathology observation, the intestines of mice treated with PHA-L recovered significantly as well. Therefore, plant lectin PHA-L can protect mice from radiation injuries, yet the mechanism needs further study.

关 键 词：辐射损伤 植物凝集素PHA-L 放射性肠炎 

分 类 号：R979.6[医药卫生—药品]