慢性心力衰竭大鼠肾组织中血小板源性生长因子及结缔组织生长因子的表达  

Expression of platelet-derived growth factor-BB and connective tissue growth factor in renal tissues of rats with chronic heart failure

作　　者：杨晓欧 张梦云[1] 李秀华[1] 冯翔宇 彭玉娟[3] 

机构地区：[1]承德医学院附属医院老年病科,承德067000 [2]沧州人民医院心血管内科,沧州061000 [3]泰安市第一人民医院心血管内科,泰安271000

出　　处：《解剖学杂志》2016年第2期161-165,共5页Chinese Journal of Anatomy

基　　金：河北省卫生计生委医学科学研究重点课题计划(20110180)

摘　　要：目的：探讨血小板源性生长因子-BB（PDGF-BB）及结缔组织生长因子（CTGF）在慢性心力衰竭大鼠肾组织的表达及意义。方法：采用肾上腹主动脉缩窄法制作Wistar大鼠慢性心力衰竭模型，随机分为假手术组，心衰10d、20d、30d组。观察及比较各组大鼠的血流动力学数值；Masson染色方法观察大鼠慢性心衰时肾组织结构变化；免疫组织化学方法检测各组大鼠肾组织的PDGF-BB及CTGF的表达部位；免疫印迹半定量检测各组大鼠肾组织的PDGF-BB、CTGF的表达水平。结果：心衰模型各组大鼠PDGF-BB、CTGF的蛋白表达均较假手术组高，且随心衰程度的加重而表达升高。结论：慢性心衰可引起肾间质纤维化，其发生发展与心衰的严重程度密切相关。Objective： To investigate the expression and significance of platelet-derived growth factor BB （PDGF-BB） and connective tissue growth factor （CTGF） in renal tissues of rats with chronic heart failure. Methods： Make a model of chronic heart failure in Wistar rats by abdominal aorta constriction. The rats were divided into four groups： the sham operation group, the heart failure 10 days group, the heart failure 20 days group and the heart failure 30 days group. Observe and compare the hemodynamic parameters and the left ventricular mass index of the four groups of rats; observe the changes of renal tissues in chronic heart failure rats by Masson method; detect the expression of PDGF-BB and CTGF in renal tissues of rats in each group by immunohistochemical method and Western blotting. Results： The expression of protein PI^F-BB and CTGF in heart failure model rats were higher than those in the sham operation group, and with the increased degree of heart failure, the more expression of PDGF-BB and CTGF. Conclusion： Chronic heart failure can induce renal interstitial fibrosis, and the severity of renal interstitial fibrosis in chronic heart failure is closely related to the severity of heart failure.

关 键 词：慢性心衰 肾肾间质纤维化 血小板源性生长因子 结缔组织生长因子 

分 类 号：R541.6[医药卫生—心血管疾病]