维吾尔族群体脂蛋白脂酶基因Hind Ⅲ多态性与血生化指标的相关性  

Correlation between Hind Ⅲ polymorphism of lipoprotein lipase gene and blood biochemistry in Uyghur population

作　　者：布帕提玛穆·阿布都克热穆 伊力哈木·乃扎木[1] 宋曼殳[2] 多力坤·买买提玉素甫[1] 热比亚木·巴克 

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046 [2]首都医科大学公共卫生与家庭医学学院,北京100069

出　　处：《解剖学杂志》2016年第2期218-221,228,共5页Chinese Journal of Anatomy

基　　金：国家自然科学基金(31460285,30901238);新疆维吾尔自治区自然科学基金(2013211A016);新疆维吾尔自治区科技支疆项目(201191247)

摘　　要：目的：探讨脂蛋白脂酶（LPL）基因HindⅢ酶切位点多态性在新疆南部维吾尔族群体中的分布及其与血生化指标的相关性。方法：采用聚合酶链反应一限制性片断长度多态性（PCR-RFLP）检测97例无血缘关系的维吾尔族健康群体LPI。基因HindⅢ酶切位点的多态性，并结合统计学软件分析其分布特点。结果：97例维吾尔族健康群体中H＋H＋、H＋H-、H—H-基因型频率分别为38．14％、57．73％、4．12％。男与女2组基因型和等位基因的分布差异无统计学意义。将总体样本分为男性组和女性组，男性组中H＋H＋基因型组总胆固醇（TC）水平显著高于H＋H-基因型组，H＋H～基因型组甘油三酯（TG），SBP水平显著高于H＋H＋基因型组；女性组中收缩压和舒张压水平在H＋H＋、H＋H-、H—H-基因型间的差异有统计学意义。维吾尔族健康群体LPL基因．HindⅢ酶切位点多态性与国内外的一些民族之间存在比较大的差异。结论：LPL基因HindⅢ酶切位点的多态性在不同种族问存在着较大的差异，这种差异可能是导致某些疾病在不同种族间的发现率和临床表现存在显著不同的因素之一。Objective： To investigate the relationship between HindⅢ polymorphism of lipoprotein lipase （LPL） gene and blood biochemistry in southern Xinjiang Uyghur population in China. Methods： The techniques of polymerase chain reaction restriction fragment length polymorphism（PCR-RFLP）was used to detect the Hind Ⅲ polymorphism of LPL gene in 99 unrelated Uyghur healthy individuals and statistics software was applied for data analysis. Results： All subjects were genotyped for LPL gene. The frequencies of the H＋H＋,H＋H- ,H＋H- genotypes were 38.14%, 57.73%, 4. 12%, respectively. Genotype frequency and allele frequency were not related to sex. The overall sample was divided into male and female groups. One-way ANOVA analysis showed that in male group, people with Hd-H＋ genotype had higher levels of total cholesterol （TC） compared with the H＋H＋ genotype group. People with H＋H＋ genotype had higher levels of triglyeride（TG） and systolic pressure compared with the H＋H＋ genotype group. In female group, levels of systolic pressure and diastolic pressure in the three genotype groups were statistically significant. There was a significant difference between the Uyghur LPL gene Hind ]I] polymorphism and some ethnic groups. Conclusion： There is a significant difference between the Uyghur LPL gene polymorphism and some ethnic groups, which may contribute to the different incidence rates and clinical performances of certain diseases in different races.

关 键 词：维吾尔族 脂蛋白脂酶基因 多态性 血生化指标 

分 类 号：R440[医药卫生—诊断学]