溶血性评价体系中分光光度法检测波长的选择  

Wavelength Selection in Hemolytic Evaluation Systems with Spectrophotometry Detection

作　　者：张红[1] 苏宝倡 叶迅达 罗曼[1] 

机构地区：[1]暨南大学生命科学技术学院,广州510632 [2]暨南大学附属第一医院输血科,广州510630

出　　处：《生物医学工程学杂志》2016年第2期368-372,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30973158);广东省教育部产学研结合资助项目(2010B090400182);广东省科技厅专项资金资助项目(2014A020208025)

摘　　要：分光光度法是对新药、植入性生物医用材料及血液制品进行溶血性评价的常用简便方法,是针对血红蛋白特征吸收峰进行的定量分析,因此,当评价体系对血红蛋白构象产生影响时,检测波长的正确选择就显得至关重要。本文通过对在细胞培养液、磷酸盐缓冲液（PBS）、生理盐水和库血保存液四种体系中红细胞溶血的上清进行连续波长扫描及其特征峰随时间变化的研究,提出如下检测波长的选择方案：细胞培养液中,4h内检测选415nm,4-72h选408nm附近;PBS中,4h内选541、577或415nm,4-72h选541、577或406nm附近;生理盐水中,4h内选414nm,4-72h选405nm附近,12h内还可选541或577nm;库血保存液中,72h内选415、540或576nm。Spectrophotometry is a simple hemolytic evaluation method commonly used in new drugs,biomedical materials and blood products.It is for the quantitative analysis of the characteristic absorption peaks of hemoglobin.Therefore,it is essential to select the correct detection wavelength when the evaluation system has influences on the conformation of hemoglobin.Based on the study of changes in the characteristic peaks over time of the hemolysis supernatant in four systems,namely,cell culture medium,phosphate buffered saline（PBS）,physiological saline and banked blood preservation solution,using continuous wavelength scanning,the selections of detection wavelength were proposed as follows.In the cell culture medium system,the wavelength of 415 nm should be selected within 4h;,near 408 nm should be selected within 4-72h.In PBS system,within 4h,541 nm,577nm or 415 nm should be selected;4-72h,541 nm,577nm or near 406 nm should be selected.In physiological saline system,within 4h,414 nm should be selected;4-72h,near 405 nm should be selected;within 12 h,541nm or 577 nm could also be selected.In banked blood preservation solution system,within 72 h,415nm,540 nm or 576 nm should be selected.

关 键 词：溶血性评价 分光光度法 红细胞 血红蛋白 库血 

分 类 号：R318.08[医药卫生—生物医学工程]