中国南方汉族人群哮喘与GSDMB/ORMDL3基因多态性  

Association of GSDMB/ORMDL3 polymorphism with asthma risk among Han population in Southern China

作　　者：胡慧婷[1] 杨帆[1] 汪亚君[1] 吴斌[1] 

机构地区：[1]广东医学院附属医院,广东湛江524001

出　　处：《中国公共卫生》2016年第4期502-506,共5页Chinese Journal of Public Health

基　　金：国家自然科学青年基金(81400023);广东省医学科研基金(A2015338)

摘　　要：目的研究GSDMB/ORMDL3基因相关单核苷酸多态性位点(SNP)与中国广东人群哮喘发病、吸烟行为的相关性。方法于2014年3月—2015年3月对广东医学院附属医院就诊的394例哮喘患者、395名健康成人进行血样采集和问卷调查,用SNa Pshot技术检测GSDMB/ORMDL 3基因单核苷酸多态性(SNP)rs7216389、rs4794820、rs11650680基因型,并分析SNP变异与哮喘风险及吸烟因素的相关性。结果 SNP位点rs7216389C-T和rs4794820 A-G变异增加哮喘发病风险(OR分别为1.8和1.7,均为P<0.000 1),而rs11650680 C-T位点变异与哮喘风险无明显相关性(OR=0.8,P=0.170 0);吸烟哮喘患者rs7216389 T/T和rs11650680 C/C高于戒烟与非吸烟组,而rs4794820 A/A低于非吸烟组(均为P<0.000 1)。结论 rs7216389 C-T、rs4794820 A-G位点变异与哮喘发病风险明显相关,吸烟增加rs7216389 C-T、rs4794820 A-G、rs11650680 T-C位点的变异风险。Objective To explore potential associations between three gasdermin B / sphingo-lipid biosynthesis regulator 3（ GSDM B / ORM DL3） genes polymorphism with asthma risk and smoking behavior in Han population in Guangdong province of China. Methods A total of 394 asthma patients and 395 healthy physical examinees recruited from Affiliated Hospital of Guangdong M edical College were genotyped to detect single nucleotide polymorphism（ SNP） of three GSDM B / ORM DL3 genes（ rs7216389,rs4794820 and rs11650680） using SNa Pshot technology. The correlations of gene polymorphism with asthma risk and smoking behavior were analyzed. Results The mean age of the asthma patients and healthy physical examinees was 45. 37 and 45. 23 years,respectively. There were no significant differences in age,gender and smoking status between the asthma patients and the healthy examinees. The variations in rs7216389 C / T and rs4794820 A / G genotype were significantly associated with increased asthma risk,with the odds ratio（ OR） of 1. 8 and1. 7（ both P〈0. 0001）; while the variation of rs11650680 C / T was significantly correlated with asthma risk（ OR = 0. 8,P = 0. 1700）. The frequency of rs7216389 T / T and rs11650680 C / C were higher among smoking asthma patients than among nonsmoking asthma patients,but the frequency of rs4794820 A / A was lower among the smoking asthma patients than among nonsmoking asthma patients significantly（ P〈0. 0001 for all）. Conclusion Our results showthat rs7216389 C / T and rs4794820 A / G variations are associated with higher asthma risk and smoking could increases variation frequency in rs7216389 C / T,rs4794820 A / G,and rs11650680 T / C of GSDM B / ORM DL3 among Han population in Guangdong province of China.

关 键 词：GSDMB/ORMDL 3 基因多态性 哮喘 吸烟 

分 类 号：R181.2[医药卫生—流行病学]