葶苈子水提液对内毒素致急性肺损伤大鼠肺泡Ⅱ型上皮细胞水通道蛋白5表达的影响  

Effects of Semen Lepidii on the Expression of Aquaporin-5 of Alveolar Cells Type Ⅱin Rats with Endotoxin Induced Acute Lung Injury

作　　者：张朝晖[1] 徐小云[1] 瞿星光[1] 邹文博[1] 李灵丰[1] 曾超[1] 姚玲[1] 钟建华[1] 

机构地区：[1]三峡大学第一临床医学院,湖北省宜昌市中心人民医院,湖北宜昌443003

出　　处：《中国中医急症》2016年第4期606-608,622,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：湖北省宜昌市科技攻关项目

摘　　要：目的观察葶苈子水提液对内毒素致急性肺损伤大鼠肺泡Ⅱ型上皮细胞水通道蛋白5表达的影响。方法清洁级SD大鼠18只,随机分成空白对照组、模型组、葶苈子治疗组(治疗组)共3组,尾静脉注射内毒素建立急性肺损伤模型后4 h处死大鼠。计量24 h尿量,收集血液样本,测定血气分析,摘取肺脏,称重并计算肺系数,HE染色法观察肺组织病理,并采用免疫电泳法测定通道蛋白5(AQP5)的表达。结果模型组、治疗组肺湿重、肺系数与空白对照组相比,肺湿重、肺系数差异均具有统计学意义(均P<0.01);治疗组肺湿重、肺系数与模型组比较,差异有统计学意义(P<0.05)。与空白对照组相比较,模型组大鼠24 h尿量有所减少,但差异无统计学意义(P>0.05);葶苈子治疗组与模型组比较,大鼠24 h尿量差异有统计学意义(P<0.01)。模型组、治疗组与空白对照组相比,Pa O2、Pa CO2、p H均值差异均具有统计学意义(均P<0.01);治疗组Pa O2、Pa CO2、p H与模型组比较,差异有统计学意义(P<0.05)。肉眼观,与对照组比较,模型组大鼠双肺充血水肿、肺外周可见片状炎性渗出、体积明显增大,肺泡呈粉红色,气管内有粉红色分泌物溢出,或见血性胸水;模型组100倍镜下示肺泡广泛水肿及片状炎细胞浸润,局灶性出血,结构轮廓几乎丧失,部分肺泡受压萎陷。肉眼观,治疗组较模型组大鼠双肺肿胀减轻,肺表面光滑,弹性可;光镜下治疗组肺泡可见充血水肿,炎细胞浸润,肺泡结构及轮廓显示不清,但较模型组明显减轻,肺泡内水肿液明显减少,仅见炎细胞浸润。电泳结果示,模型组APQ5蛋白表达较对照组明显下调(P<0.01),与模型组比较,葶苈子治疗组可明显上调APQ5蛋白表达。结论葶苈子有改善急性肺损伤的效果,这种作用可能与其改善水通道蛋白5含量或者活性有关。Objective：To observe the effect of semen lepidii on the expression of aquaporin-5 of alveolar cells type Ⅱin rats with endotoxin induced acute lung injury.Methods：18 Clean SD rats were randomly divided into the control group,the model group and lepidii group.The model of acute lung injury was established with tail vein injection of endotoxin,and the rats were executed 4 hours after successful modeling.24 hours urine output was measured.The blood was collected and blood gas analysis was detected.Both lungs were removed and weighed.The lung coefficient was calculated.HE staining was used to observe the pathological changes of lung tissue and the expression of AQP5 was detected with the method of immuno electrophoresis.Results：Compared with the control group,there was statistical significance in the level of lung wet weight and lung coefficient of the model group and lepidii group（P〈0.01）,as well as in the comparison of lepidii group and the model group（P〈0.05）.Compared with the control group,24 hours urine output of the model group decreased a little,without statistical significance（P〉0.05）.However,it was opposite in the comparison between the lepidii group and the model group（P〈0.01）.The mean difference in PaO_2,PaCO_2 and PH among the three groups had statistical significance（P〈0.01）,as well as in the comparison of lepidii group and the model group（P〈0.05）.Gross appearance showed that compared with the control group,there was congestion and edema in both lungs and patchy inflammatory exudation in peripheral lung in the model group;volume increased significantly with pink alveolar,pink secretion from airway and maybe bloody pleural fluid.100 times under the light microscope,there was alveolar edema,patchy inflammatory exudation and focal hemorrhages;structure outlines were almost lost;part of alveolar collapsed because of pressure.Gross appearance showed that compared with the model group,edema in both lungs relieved with smooth surface and resilience.Under the light mic

关 键 词：急性肺损伤 葶苈子提取液 水通道蛋白 

分 类 号：R285.5[医药卫生—中药学]