疏肝和胃方对胃食管反流大鼠模型背根神经节中TRPV1表达的影响  

Influence of Shugan Hewei Decoction on TRPV1 in Dorsal Root Ganglia of Rats with Reflux Esophagitis

作　　者：曹会杰[1] 刘春芳[2] 程艳梅[2] 张秀莲[2] 王宏伟[2] 朱生樑[2] 

机构地区：[1]上海中医药大学附属普陀医院,上海200002 [2]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《中国中医急症》2016年第4期623-626,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家中医药管理局"十二五"重点专科项目(No.09JIX1L206B118);上海中医药大学附属岳阳中西医结合医院科研基金项目(No.30304115238)

摘　　要：目的观察疏肝和胃方对胃食管反流大鼠模型背根神经节中内脏敏感因子瞬时受体电位香草酸亚型1(TRPV1)表达的影响,探讨其治疗胃食管反流病的机理。方法将40只SD大鼠按随机数字表法分为正常组、模型组、中药组及西药组,每组10只,除正常组外均行贲门肌切开术加幽门半结扎术制备反流性食管炎模型,并分别给予相应干预措施。免疫组化法及实时荧光定量核酸扩增检测(QPCR)方法观察各组大鼠背根神经节TRPV1受体及TRPV1 m RNA的表达。结果与正常组相比,模型组、西药组大鼠背根神经节中TRPV1阳性表达明显增高(P<0.05);中药组与正常组比较无统计学差异(P>0.05);与西药组比较,中药组TRPV1阳性表达明显降低(P<0.05)。模型组背根神经节组织中TRPV1 m RNA表达水平明显高于正常组、中药组及西药组(P<0.05);中药组、西药组与正常组比较差异无统计学意义(P>0.05);中药组与西药组比较,两组背根神经节组织中TRPV1 m RNA表达差异无统计学意义(P>0.05)。结论疏肝和胃方可能通过抑制TRPV1过度表达来发挥其治疗胃食管反流病的作用。Objective：To observe the influence of Shugan Hewei Decoction on TRPV1 in dorsal root ganglia of rats with reflux esophagitis and explore the mechanism of the treatment of gastroesophageal reflux disease.Methods：Fourty healthy male Spregue-Dawley（SD）rats were randomly divided into 4 groups（n = 10）：the normal group,the model group,TCM group,Western medicine group.Reflux esophagitis models were induced by cardiomyotomy and pylorus semiligation,and given the corresponding intervention measures.Expression of TRPV1 receptors and the expression of TRPV1 m RNA in dorsal root ganglia of rats were observed with immunohistochemical method and QPCR.Results：Compared with the normal group,positive expressions of TRPV1 in model group and Western medicine group were significantly higher（P〈0.05）.There was no significant difference between the treatment group and TCM group（P〉0.05）.Compared with Western medicine group,the positive expression of TRPV1 was significantly decreased in TCM group;the difference was statistically significant（P〈0.05）.The expression level of TRPV1 m RNA in model group was significantly higher than normal group,TCM group and Western medicine group（P〈0.05）.The difference was not statistically significant between normal group,TCM group and Western medicine group（P〉0.05）.The expression of TRPV1 m RNA in the last two groups was not statistically significant（P〉0.05）.Conclusion：The mechanism of Shugan Hewei Decoction on gastroe sophageal reflux disease may inhibitting the overexpression of TRPV1.

关 键 词：胃食管反流病 反流性食管炎 瞬时受体电位香草酸亚型1(TRPV1) 疏肝和胃方 

分 类 号：R285.5[医药卫生—中药学]