桃红芩连汤对脓毒症大鼠心肌高迁移率族蛋白B1的影响  

Effect of Taohong Qinlian Decoction on HMGB1 in Septic Rat Cardiac Muscle

作　　者：顾忠民[1] 林绍彬[1] 刘才俊[1] 齐心欣[1] 

机构地区：[1]厦门大学附属福州市第二医院重症医学科,福州350007

出　　处：《中国中西医结合杂志》2016年第4期445-448,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：福建省科技厅2014重点项目(No.2014D029);福州市科技局项目(No.2013-S-123-1)

摘　　要：目的观察脓毒症大鼠心肌高迁移率族蛋白B1(HMGB1)、血清肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)、肌钙蛋白Ⅰ(TnⅠ)的水平,探讨桃红芩连汤治疗脓毒症心肌损伤机制。方法 48只清洁级健康雄性Wistar大鼠随机分成假手术组(Sham组)、脓毒症模型组(CLP组)、脓毒症中药治疗组(ZY组),每组16只。于手术后24、48h分别检测TNF-α、IL-6、TnⅠ、HMGB1浓度,光镜观察心肌病理变化。结果与Sham组比较,CLP组在24、48hTNF-α、IL-6、TnⅠ及HMGB1水平均显著升高,差异有统计学意义(P<0.01);与CLP组同期比较,ZY组24、48h时TNF-α、IL-6、TnⅠ及HMGB1浓度均明显下降,差异亦有统计学意义(P<0.05)。光镜下CLP组和ZY组可见心肌损伤,CLP组较ZY组损伤明显。结论桃红芩连汤可降低脓毒症相关炎症因子水平,对心肌有保护作用。Objective To observe the levels of high mobility group box-1 protein（HMGB1）,tumor necrosis factor-alpha（TNF-α）,IL-6,troponin Ⅰ（TnⅠ）release in septic rats,and to explore the mechanism of Taohong Qinlian Decoction（TQD）in the treatment of septic myocardial injury.Methods A total of 48 healthy male Wistar rats of clean grade were randomly divided into the sham-operation group（Sham）,the sepsis model group（CLP）,and the TQD treatment group（ZY）,16 in each group.Concentrations of TNF-α,IL-6,TnⅠ,and HMGB1 expression were detected in each group at 24 and 48hafter operation.Pathological changes of cardiac muscle were observed under light microscope.Results Concentrations of TNF-α,IL-6,TnⅠ and HMGB1 at 24and 48 hafter operation were significantly higher in the CLP group than in the Sham group（P〈0.01）.Concentrations of TNF-α,IL-6,TnⅠ,and HMGB1 at 24and 48 hafter operation were significantly lower in the ZY group than in the CLP group（P〈0.05）.Myocardial injury occurred in the CLP and the ZY group under light microscope.And this injury was more severe in the CLP group than in the ZY group.Conclusion TQL could reduce the level of sepsis-related inflammatory cytokines and protect myocardium in septic rats.

关 键 词：桃红芩连汤 脓毒症 心肌细胞 高迁移率族蛋白B1 

分 类 号：R285.5[医药卫生—中药学]