银杏叶片对动脉粥样硬化性大鼠主动脉壁清道夫受体A表达的影响  

Effect of Ginkgo biloba Tablet on the Expression of Scavenger Receptor A of the Aortic Wall in Atherosclerotic Rats

作　　者：朱贵月[1] 朱伟[2] 潘凌云[1] 马晓静[1] 苑海涛[1] 杨光[3] 

机构地区：[1]山东大学附属省立医院心内科,济南250021 [2]山东省医学科学院放射研究所,济南250012 [3]山东大学附属千佛山医院保健科,济南250014

出　　处：《中国中西医结合杂志》2016年第4期449-453,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：山东省自然科学基金课题项目(No.Q2007C10)

摘　　要：目的观察银杏叶片对动脉粥样硬化性大鼠主动脉壁清道夫受体A的表达及血清炎性因子变化的影响,探讨银杏叶片抗动脉粥样硬化斑块形成的新机制。方法 Wistar雄性大鼠45只,随机分成对照组、模型组及银杏叶片组。测定各组大鼠血糖、血脂、血钙、血清C-反应蛋白(C-reactive protein,CRP)、可溶性细胞间黏附分子-1(soluble intercellular adhesion molecule-1,sICAM-1)和可溶性血管细胞黏附分子-1(soluble vascular cell adhesion molecule-1,sVCAM-1)水平,免疫组化法观察清道夫受体A在动脉壁表达的情况,并观察清道夫受体A表达与炎症因子水平的相关性。结果与对照组比较,模型组血糖、血钙明显升高(P<0.05),甘油三酯(TG)、血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平显著升高(P<0.01);新生内膜面积显著增厚,内膜增生百分比显著变大,管腔狭窄指数显著变小,差异均有统计学意义(P<0.01);CRP、sICAM-1、sVCAM-1水平显著升高(P<0.01)。与模型组比较,银杏叶片组血糖、血钙明显降低(P<0.05),TG、TC、LDL-C水平显著降低(P<0.01)。模型组主动脉管腔比银杏叶片组明显狭窄(P<0.05)。清道夫受体A在动脉壁有表达,而银杏叶片组较模型组3项指标显著改善,差异均有统计学意义(P<0.01)。银杏叶片组较模型组可显著降低动脉粥样硬化大鼠血清CRP、sICAM-1、sVCAM-1水平(P<0.01)。血清炎症因子(CRP、sICAM-1、sVCAM-1)水平与动脉壁清道夫受体A阳性表达面积百分比呈正相关(r分别为0.701、0.604、0.581,均P<0.01)。结论动脉粥样硬化大鼠血清中炎症因子水平增高、动脉壁清道夫受体A表达增强,且清道夫受体A表达与炎症因子水平密切相关。银杏叶片可降低炎症因子水平、抑制清道夫受体A表达。Objective To observe the expression of Ginkgo biloba Tablet（GbT）on scavenger receptor A（SRA）of the aortic wall and changes of serum inflammatory factors in atherosclerotic rats,and to explore its new mechanism for fighting against atherosclerosis（AS）.Methods Totally 45 male Wistar rats were randomly divided into the control group,the model group,the GbT group,15 rats in each group.Levels of blood glucose,blood lipids,blood calcium,serum C-reactive protein（CRP）,soluble intercellular adhesion molecule-1（sICAM-1）,and soluble vascular cell adhesion molecule-1（sVCAM-1）were measured in all rats.The expression of SRA in the aortic wall of atherosclerotic rats was observed by immunohistochemical assay.The correlation between the expression of SRA andlevels of inflammatory factors was also observed.Results Compared with the control group,blood glucose and blood calcium obviously increased（P〈0.05）;levels of TG,TC,and LDL-C were significantly elevated（P〈0.01）;neointimal areas were significantly thickened,increased intima percentage was significantly enlarged,narrowed lumen index was significantly reduced;levels of CRP,sICAM-1,and sVCAM-1 were significantly elevated in the model group（all P〈0.01）.Compared with the model group,blood glucose and blood calcium obviously decreased（P〈0.05）;levels of TG,TC,and LDL-C significantly decreased（P〈0.01）in the GbT group.Aortic lumens were obviously narrower in the model group than in the GbT group（P〈0.05）.SRA expressed at the aortic wall.The aforesaid 3 indices were significantly improved in the GbT group than in the model group（P〈0.01）.Serum levels of CRP,sICAM-1,and sVCAM-1 were significantly decreased in the GbT group than in the model group（P〈0.01）.Serum levels of CRP,sICAM-1,and sVCAM-1 were positively correlated with the percentage of SRA positive expression area（r=0.701,0.604,0.581,all P〈0.01）.Conclusions Serum levels of inflammatory factors in atherosclerotic rats were elevated,and the expression of SRA

关 键 词：银杏叶片 动脉粥样硬化性大鼠 清道夫受体A C-反应蛋白 可溶性细胞间黏附分子-1 可溶性血管细胞黏附分子-1 

分 类 号：R285.5[医药卫生—中药学]