芩丹颗粒对微波辐射大鼠POMC衍生肽含量及海马CA1区结构的影响  

Effect of Qindan Granule on POMC Derived Peptide Content and Structure of Hippocampal CA1 Region in Microwave Radiated Rats

作　　者：管兰芳[1] 李亚巍[2] 徐俊杰[3] 钟秀宏[4] 张红[3] 朱文赫[3] 吕士杰 

机构地区：[1]吉林医药学院教务处,吉林132013 [2]吉林医药学院药学教研室,吉林132013 [3]吉林医药学院生化教研室,吉林132013 [4]吉林医药学院病理教研室,吉林132013

出　　处：《中国中西医结合杂志》2016年第4期471-475,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：吉林省卫生厅科技计划项目(No.2012Z066;No.2013Q030);吉林市科技计划项目(No.201262508)

摘　　要：目的探讨高强度微波辐射对大鼠下丘脑—垂体—肾上腺轴(hypothalamo-pituitary-adrenal axis,HPAA)活动和海马CA1区结构的影响及芩丹颗粒对辐射损伤大鼠的保护作用。方法 Wistar大鼠48只,随机分为正常对照组、照射后1、7、10d组、预防给药7、10d组、治疗给药7、10d组,共计8组,每组6只。预防组首先分别给予芩丹颗粒药液1mL/100g体重(4.75g生药)灌胃7d和10d,然后给予照射,再与正常对照组、照射后1d组一起统计药物对辐射损伤的预防效果;治疗组先进行照射,然后分别给予芩丹颗粒药液(用量同预防组)灌胃7d和10d,再与正常对照组、照射后7d和10d组一起统计药物对辐射损伤的治疗效果。检测各组大鼠促肾上腺皮质激素释放激素(corticotropin releasing hormone,CRH)、β-内啡肽(β-endorphin,β-EP)、促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)和热休克蛋白70(heat shock protein 70,HSP70)含量,光镜下观察海马CA1区神经元形态结构变化。结果与正常对照组比较,各照射组大鼠垂体CRH、β-EP含量明显降低,照射后1、7d组血清ACTH和β-Ep含量显著升高(P<0.05)。与照射组比较,预防组垂体、血清β-EP含量显著增加,血清ACTH含量显著降低(P<0.05);治疗组垂体CRH、β-EP含量显著增加,治疗给药7d组血清ACTH、β-EP和HSP70含量比照射后7d组显著降低(P<0.05)。形态学观察,照射组海马CA1区锥体神经元排列紊乱,细胞肿胀,胞核变小、浓缩,胞质深染,结构不清,部分区域血管扩张瘀血,组织水肿、疏松;预防组和治疗组海马CA1区组织病理损伤明显减轻,神经元排列较规则,肿胀、固缩或脱失神经元数目较少,血管扩张充血减轻。结论芩丹颗粒可以影响高强度微波辐射大鼠HPAA功能活动,调整HPAA部分生物活性肽及激素的合成与释放,改善海马CA1区组织病理损伤程度,对微波辐射损伤大鼠具有一定的预防和治疗作用。Objective To explore the protection of high intensity microwave radiation on hypothalamo-pituitary-adrenal axis（HPAA）activity and hippocampal CA1 structure in rats and the protective effect of Qindan Granule（QG）on radiation injured rats.Methods Totally 48 Wistar rats were randomly divided into 8 groups,i.e.,the normal control group,post-radiation day 1,7,and 10 groups,7 and 10 days prevention groups,day 7 and 10 treatment groups,6in each group.Rats in prevention groups were respectively administered with QG liquid（1 mL/100 g,4.75 g crude drugs）for 7 days and10 days by gastrogavage and then microwave radiation.Then preventive effect for radiation injury was statistically calculated with the normal control group and the post-radiation day 1 group.Rats in treat-ment groups were firstly irradiated,and then administered with QG liquid（1 mL/100 g,4.75 g crude drugs）.Finally preventive effect for radiation injury was statistically calculated with the normal control group,post-radiation day 7and 10 groups.Contents of corticotrophin releasing hormone（CRH）,beta endorphin（beta-EP）,adrenocorticotropic hormone（ACTH）,and heat shock protein70（HSP70）were detected.Morphological changes and structure of hippocampal CA1 region were observed under light microscope.Results Compared with the normal control group,contents of CRH and beta-EP significantly decreased in each radiation group.Serum contents of ACTH and beta-EP significantly increased in post-radiation day 1 and 7 groups（P〈0.05）.Compared with radiation groups,beta-EP content in serum and pituitary significantly increased,and serum ACTH content significantly decreased in prevention groups（P〈0.05）.Pituitary contents of CRH and beta-EP significantly increased in prevention groups.Serum contents of ACTH,beta-EP,and HSP70 were significantly lower in day 7 treatment group than post-radiation day 7 group（P〈0.05）.Morphological results showed that pyramidal neurons in the hippocampal CA1 region arranged in disorder,with swollen cells,

关 键 词：微波辐射 芩丹颗粒 促肾上腺皮质激素 海马 

分 类 号：R285.5[医药卫生—中药学]