采用DNA条形码技术检测马铃薯4种细菌病害  

Detection of Four Bacterial Diseases of Potato by DNA Barcoding

作　　者：魏琪[1] 闵凡祥[1] 张抒[1] 高云飞[1] 董学志[1] 王文重[1] 杨帅[1] 

机构地区：[1]黑龙江省农业科学院植物脱毒苗木研究所,黑龙江哈尔滨150086

出　　处：《中国马铃薯》2016年第2期105-111,共7页Chinese Potato Journal

基　　金：黑龙江省青年科学基金项目(QC2012C063);现代农业产业技术体系专项资金资助(CARS-10-P14);国际合作项目(2013DFA31970);国家科技支撑项目(2012BAD06B02);公益性行业科研专项经费项目(201303015-9)

摘　　要：马铃薯易受到多种细菌病害的侵染,特别是检疫性病害和土传性病害,对马铃薯种薯进行全面的细菌病害检测势在必行。将检测检疫性细菌病害的DNA条形码技术应用在马铃薯4种细菌性病害(环腐病、青枯病、疮痂病和黑胫病)的检测中,探讨该项技术的应用可行性。采用已知菌株以明确该项技术的应用效果,结果表明,该项DNA条形码技术检测马铃薯环腐病和疮痂病的结果良好;检测青枯病可以确定到属;检测黑胫病时配合特异性基因,可获得良好的检测结果。该DNA条形码技术是标准性操作规程与测序技术相结合的一种方法,检测结果的准确性高,同时可以在大规模样品的检测工作中缩减工作量,提高检测效率。Potato is vulnerable to infection by a variety of bacterial diseases, especially quarantine diseases and soil-borne diseases, and to advance a comprehensive detection of bacterial diseases for seed potato bacterial disease is imperative. In this research, four bacterial diseases of potato, including potato ring rot, bacterial wilt, potato common scab, and black leg, were detected using DNA barcoding technique to investigate the application feasibility of the technology for potato bacterial disease testing. Known strains were used to assess the detection results. The DNA barcoding technique was good for potato ring rot and potato scab detection. Bacterial wilt was determined to genus level. Black leg could also be detected by combination of DNA barcode technology and specific gene. The DNA barcoding technique includes a standard method of operating procedures, and sequencing technology, which could achieve high accuracy of testing, reduce the workload of large-scale work in potato bacterial diseases testing, and improve the detection efficiency.

关 键 词：DNA条形码技术 马铃薯 环腐病 青枯病 疮痂病 黑胫病 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]