转录因子Brn3a及胰岛因子1在三个时期中三叉神经节的表达情况  

Expression of transcription factor Brn3a and Isl-1 in trigeminal ganglia during three different periods

作　　者：王瑶[1] 姬晓炜[2] 贺瑞[3] 张琼[3] 张远[3] 王进涛[3] 张鹏涛[3] 张蕾[3] 史芳川[3] 钟良军[3] 

机构地区：[1]新疆医科大学第一附属医院牙周黏膜科,新疆乌鲁木齐830000 [2]新疆医科大学第一附属医院修复科,新疆乌鲁木齐830000 [3]杭州师范大学附属医院口腔科,浙江杭州310000

出　　处：《口腔医学》2016年第4期294-298,共5页Stomatology

基　　金：国家自然科学基金(81570989)

摘　　要：目的探讨转录因子Brn3a及胰岛因子1(Islet1,Isl-1)转录因子在生长发育的三个时期中三叉神经节中的表达情况。方法取发育不同时期的小鼠胚胎头部第13.5天、15.5天、17.5天,各个时期为相互对照。用免疫荧光的方法标记相关蛋白,观察比较不同时期的Brn3a及Isl-1在三叉神经节的表达情况。结果三个时期中Brn3a及Isl-1在三叉神经节均有共表达,在生长发育早期Isl-1没有明显增殖,在生长发育晚期Brn3a没有明显凋亡。结论在发育后期Brn3a及Isl-1标记的神经元,依旧共表达于神经元中,且随生长发育,标记Brn3a及Isl-1的神经元逐渐减少。Brn3a及Isl-1标记的神经元,在生长发育过程中分化为亚型神经元。Objective To investigate the expression of transcription factor Brn3 a and Isl-1 in trigeminal ganglion during the three periods of growth and development. Methods Mouse brains at 13. 5,15. 5 and 17. 5 days after being born were used for contrast. The immunofluorescence technique was applied to investigate the expression patterns of Brn3 and Isl-1 in trigeminal ganglion between different periods. Results In all three periods,Brn3 a and Isl-1 were co-expressed in the trigeminal ganglia. In the early stage of growth and development,Isl-1 showed no significant proliferation,and in the late stage of growth and development,Brn3 a showed no significant apoptosis. Conclusion In the late stage of development,Brn3 a and Isl-1 marked neurons were still expressed in the neurons,and with the growth and development,the marked Brn3 a and Isl-1 neurons gradually decreased. Besides,during the process of growth and development,Brn3 a and Isl-1 marked neurons differentiated into subtypes of neurons,which regulated the upstream and downstream transcription factors.

关 键 词：Brn3a Isl-1 三叉神经节 

分 类 号：R393[医药卫生—基础医学]