白芍提取物通过多巴胺D2受体治疗大鼠高泌乳素血症  

White paeony root aqueous extract treats hyperprolactinemia rats via dopamine D2 receptor

作　　者：金泽祥 王雄 

机构地区：[1]武汉市第三医院药学部,湖北武汉430060

出　　处：《中成药》2016年第4期741-745,共5页Chinese Traditional Patent Medicine

摘　　要：目的研究白芍水提取物对高泌乳素血症大鼠及体外细胞的药效学作用。方法注射盐酸甲氧氯普胺注射液制备高泌乳素血症大鼠,观察白芍提取物对模型大鼠血清泌乳素(PRL)、雌二醇(E_2)、孕酮(P)、促卵泡激素(FSH)和黄体生成素(LH)水平的影响。研究白芍提取物对大鼠垂体瘤(MMQ)细胞、垂体腺瘤(GH3)细胞和嗜铬细胞瘤(PC12)细胞株的PRL分泌、多巴胺D2受体和多巴胺转运体(DAT)的影响。结果与模型组比较,高[28.8 g/(kg·d)]、中剂量[14.4 g/(kg·d)]的白芍提取物能显著降低高泌乳素血症大鼠的PRL水平(P<0.01)。在给药24 h后,与正常组比较,5 mg/m L和10 mg/m L的白芍提取物显著抑制了MMQ细胞PRL的分泌(P<0.01),但并不影响缺乏多巴胺D2受体表达的GH3细胞PRL的分泌;4、8 mg/m L的白芍提取物显著增加了PC12细胞多巴胺D2受体和DAT的表达(P<0.05,P<0.01)。结论白芍提取物介由多巴胺D2受体治疗高泌乳素血症,且疗效显著。AIM To investigate the anti-hyperprolactinemia（ anti-hyper-PRL） activities of white paeony root aqueous extract（ WPRAE） in vivo and in vitro. METHODS Metocolopramide Dihydrochloride Injection was used to establish rat model of hyperprolactinemia. The effects of WPRAE on the levels of serum prolactin（ PRL）,estradiol（ E2）,progesterone（ P）,follicle-stimulating hormone（ FSH）,and luteinizing hormone（ LH） in hyperPRL rats were investigated. The effects of WPRAE on PRL secretion,dopamine D2 receptor and dopamine transporters（ DAT） were studied in MMQ,GH3 and PC12 cells,respectively. RESULTS Compared with the model group,WPRAE high-dose [28. 8 g/（ kg·d） ] and middle-dose [14. 4 g/（ kg·d） ] groups reduced PRL level in rats with hyper-PRL significantly. In MMQ cells,the treatments with 5 mg/m L and 10 mg/m L WPRAE significantly suppressed PRL secretion and its synthesis in 24 h compared with the control group. Consistent with a D2-action,WPRAE did not affect PRL level in rat pituitary lactotropic tumor-derived GH3 cells that were lack of the D2 receptor expression compared with the control group,but 4 mg/m L and 8 mg/m L WPRAE significantly increased the expressions of D2 receptors and DAT in PC12 cells（ P〈0. 05,P〈0. 01）. CONCLUSION WPRAE shows anti-hyper-PRL activity and its effect is through dopamine D2 receptor.

关 键 词：白芍提取物 盐酸甲氧氯普胺注射液 大鼠 高泌乳素血症 多巴胺D2受体 

分 类 号：R285.5[医药卫生—中药学]