清火栀麦胶囊HPLC特征图谱的建立和3种成分的测定  

Establishment of the HPLC specific chromatogram of Qinghuo Zhimai Capsules and determination of three constituents

作　　者：苏建 刘永利 

机构地区：[1]河北省药品检验研究院,河北石家庄050011

出　　处：《中成药》2016年第4期810-815,共6页Chinese Traditional Patent Medicine

基　　金：河北省中医药管理局科技计划项目(2014182)

摘　　要：目的建立清火栀麦胶囊（穿心莲、栀子）HPLC特征图谱,并测定栀子苷、穿心莲内酯、脱水穿心莲内酯的含有量。方法分析采用C（18）色谱柱（250 mm×4.6 mm,5μm）;乙腈-水为流动相,梯度洗脱;体积流量1 m L/min;检测波长225 nm（穿心莲内酯、脱水穿心莲内酯）、238 nm（栀子苷）。结果清火栀麦胶囊HPLC特征图谱中有9个主要色谱峰。栀子苷、穿心莲内酯、脱水穿心莲内酯分别在0.020～2.022μg、0.016～6.431μg、0.019～7.577μg范围内线性关系良好,平均回收率（n=9）分别为98.3%（RSD=1.8%）、97.2%（RSD=1.7%）、99.7%（RSD=2.4%）。结论该方法准确可靠,可用于清火栀麦胶囊的质量控制。AIM To establish the HPLC specific chromatogram of Qinghuo Zhimai Capsules（ Andrographis paniculata and Gardenia jasminoides） and to determine the contents of geniposide,andrographolide and dehydroandrographolide. METHODS The analysis was performed on C（18）column（ 250 mm × 4. 6 mm,5 μm）,mobile phase was acetonitrile-water in a gradient elution manner at a flow rate of 1 m L/min,and detection wavelengths were set at 225 nm for andrographolide and dehydroandrographolide,and 238 nm for geniposide. RESULTS There were nine main chromatographic peaks in the HPLC specific chromatogram of Qinghuo Zhimai Capsules. Geniposide,andrographolide and dehydroandrographolide showed good linear relationships within the ranges of0. 020- 2. 022 μg,0. 016- 6. 431 μg and 0. 019- 7. 577 μg,whose average recoveries（ n = 9） were 98. 3%,97. 2% and 99. 7% with the RSDs of 1. 8%,1. 7% and 2. 4%,respectively. CONCLUSION This method is accurate and stable,which can be used for the quality control of Qinghuo Zhimai Capsules.

关 键 词：清火栀麦胶囊 HPLC特征图谱 栀子苷 穿心莲内酯 脱水穿心莲内酯 

分 类 号：R927.2[医药卫生—药学]