血糖波动对糖尿病胃轻瘫大鼠Cajal间质细胞的影响及其机制研究  

Effects of blood glucose fluctuations on Cajal interstitial cells of rats with diabetic gastroparesis an its mechanistic studies

作　　者：王艳[1] 黄萍[1] 廖淑金[1] 

机构地区：[1]广州医科大学附属市一人民医院老年病科内分泌专科,广州510180

出　　处：《广州医药》2016年第2期1-3,共3页Guangzhou Medical Journal

摘　　要：目的探讨糖尿病胃轻瘫大鼠不同血糖水平对Cajal间质细胞(ICC)的影响及其机制。方法选择雌性Wista大鼠60只进行随机分组,实验组40只,对照组20只。实验组糖尿病Wista大鼠模型以单次腹腔注射链脲佐菌素法诱导。免疫组织化学荧光染色检测不同血糖浓度大鼠胃ICC数量及网络结构。结果实验组大鼠血糖浓度高于对照组,ICC数量,低于对照组,且比较差异有统计学意义(P<0.05)。实验组大鼠中血糖浓度越高,ICC数量越低,说明血糖浓度升高可能与平滑肌及神经末梢之间缝隙连接的减少及其ICC网络的超微结构损伤及异常有关。结论DM小鼠胃组织中血糖水平的升高,可能是DM胃中ICC数量减少的原因;外源性降低血糖能改善DM相关的胃肠道ICC病变。Objective To observe the effects of glucose fluctuations on Cajal interstitial cells（ ICC） of rats with diabetic gastroparesis（ DGP） and its mechanistic. Methods 60 Wistar rats were selected and randomly divided into two groups.20 rats in experimental group and 40 rats in control group. Used immunofluorescence staining to detect the amount of gastric ICC and network structure in DGP rats with different glucose levels. Results The blood glucose concentration in the experimental group was significantly higher than that in the control group,the amount of ICC in the experimental group was significantly lower than that in the control group（ P〈0. 05）. The amount of ICC decreased with the increase of glucose levels. In the experimental group,The gap junctions between smooth muscle and nerve endings,ultrastructural damage and abnormalities of the ICC network were probably related to glucose level. Conclusion The increase of glucose level was probably the cause of the decrease of the amount in ICC. Exogenousy decrease glucose levels probably can help to improve the lesion of ICC with DGP.

关 键 词：血糖 糖尿病 胃轻瘫 胰岛素 CAJAL间质细胞 

分 类 号：R587.2[医药卫生—内分泌]