小脑延髓池注射纳洛酮对心肺复苏大鼠脑组织c-Fos mRNA及蛋白表达的影响  

Effects of naloxone injected into cisterna magna on expression of c-Fos proteins and c-Fos mRNA in brain tissues of rats following cardiopulmonary resuscitation

作　　者：朱明辉[1] 陆婉晖[1] 黎靖麟[1] 成秋生[1] 胡广奋[1] 刘四红[1] 邓穗德[1] 喻宁芳[1] 

机构地区：[1]广州市第一人民医院急诊内科,广州510180

出　　处：《广州医药》2016年第2期4-7,共4页Guangzhou Medical Journal

基　　金：广东省科技计划项目(2013B022000028)

摘　　要：目的探讨小脑延髓池注射纳洛酮对心肺复苏大鼠脑神经保护的作用机制。方法将30只雄性SD大鼠随机分为假手术组、常规复苏组和纳洛酮复苏组。采用窒息法建立大鼠心脏骤停模型,复苏的同时给予药物治疗。恢复自主循环(ROSC)后24 h取脑组织,荧光定量PCR法检测脑组织c-Fos mRNA表达水平,免疫组化法检测脑组织c-Fos蛋白的表达。结果与常规复苏组比较,纳洛酮可显著降低大鼠脑组织c-Fos mRNA及蛋白表达量(P<0.01)。结论小脑延髓池注射纳洛酮可及时有效的作用于c-Fos基因,发挥脑神经保护作用。Objective To investigate the neuroprotective mechanism of naloxone injected into cisterna magna on cerebral ischemia-reperfusion. Methods Thirty adult male SD rats were randomly divided into sham group,conventional cardiopulmonary resuscitation（ CPR） group and naloxone CPR group. Asphyxiation was used to set up rat cardiac arrest model,and corresponding drugs were given when the resuscitation was carried out. The Brain tissues were taken at 24 h after restoration of spontaneous circulation（ ROSC）. Fluorescence quantitative polymerase chain reaction（ PCR） and immunohistochemical was used to detect the expression of c-Fos proteins was used to detect the expression of c-Fos mRNA level. Results Compared with the conventional CPR group,Naloxone could significantly decrease the expression of c-Fos protein and c-Fos mRNA in rat brain.Conclusion Naloxone injected into cisterna magna can promptly and effectively act on c-Fos gene,playing a neuroprotective role.

关 键 词：小脑延髓池 纳洛酮 心肺复苏 脑缺血再灌注 C-FOS基因 

分 类 号：R459.7[医药卫生—急诊医学]