哇巴因对大鼠离体心功能作用的量-效关系  

Dose-effect relationship of quabain on the cardiac function of isolated rat heart

作　　者：王海珍[1] 史娜[1] 王耀军[2] 王竹青[2] 李盼盼[2] 姜玉新[1] 王静[1] 周萍萍[1] 王海华[1] 

机构地区：[1]皖南医学院生理教研室,安徽芜湖241002 [2]皖南医学院临床学院,安徽芜湖241002

出　　处：《皖南医学院学报》2016年第2期108-112,共5页Journal of Wannan Medical College

基　　金：安徽省高校省级自然科学研究重点项目(KJ2013A251;KJ2016A729);安徽省级大学生创新创业训练计划项目(AH201310368107);皖南医学院大学生科研基金资助项目(WK2013S22);国家级大学生创新创业训练计划项目(201510368009)

摘　　要：目的:观察不同浓度哇巴因(ouabain,OUA)对大鼠离体心功能的影响。方法:SD雄性大鼠随机分成7组(n=6):正常组(NC组),OUA 10^(-9)mol/L组,OUA 10^(-8)mol/L组,OUA 10^(-7)mol/L组,OUA 10^(-6)mol/L组,OUA 10^(-5)mol/L组,OUA 10^(-4_mol/L组。左心室插管检测各组大鼠离体心脏血流动力学指标:左室峰压(LVSP)、心率(HR)、左室内压最大上升速率(+dp/dt_(max));观察各组大鼠的心律失常发生情况并进行心律失常评分;检测各组相同时间点的冠脉流出液中乳酸脱氢酶(LDH)的活性,同时测定各组心肌组织中Na^+-K^+-ATP酶的活性,HE染色观察各组大鼠心肌形态学变化。结果:同NC组相比,OUA10^(-9)mol/L组大鼠心脏LVSP×HR和+dp/dt_(max)无明显差异(P>0.05),OUA 10^(-8)mol/L和10^(-7)mol/L组大鼠心脏LVSP×HR和+dp/dt_(max)呈剂量升高而增强(P<0.05或P<0.01);OUA 10^(-9)mol/L、OUA 10^(-8)mol/L组的Na^+-K^+-ATP酶的活性明显升高(P<0.05或P<0.01);同NC组相比,OUA浓度在(10^(-6)~10_(-4))mol/L时,OUA对各组大鼠的心功能呈剂量依赖性抑制作用(P<0.01);各组大鼠的心律失常评分明显升高;各时间点心脏灌流液中LDH的活性亦明显升高,各组心肌组织Na^+-K^+-ATP酶活性均呈剂量依赖性降低(P<0.05或P<0.01)。结论:低浓度哇巴因[(10_(-9)~10^(-7))mol/L]对大鼠离体心脏有强心作用,但随着哇巴因浓度的升高[(10^(-6)~10^(-4))mol/L],转为对心脏呈剂量依赖性抑制作用。Objective：To observe the effect of different dosage of ouabain（OUA） on the isolated cardiac function in rats.Methods：Forty-two SD rats were randomized into 7 groups（n = 6 for each）,namely：normal control group（NC group）,OUA 10-9 mol/L group）,OUA 10-8 mol/L group,OUA 10-7mol/L group,OUA 10 -6 mol/L group,OUA 10-5 mol/L group and OUA 10-4 mol/L group.Cardiac function index were evaluated by left ventricular systolic pressure（LVSP）,heart rate（HR）,the maximum upstroke velocity of intraventricular pressure（＋ dp/dtmax）.The incidence of ventricular arrhythmias and arrhythmia score were determined.Coronary effluent was collected for estimating the activity of lactate dehydrogenase（LDH）.The Na＋-K＋-ATPase content in myocardial tissues was determined,and myocardial injury was evaluated by H＆E staining.Results：As compared with the NC group,The LVSP×HR and ＋ dp/dtmax on the OUA 10-9 mol/L group had no significant difference（P 〉 0.05）,while the LVSP × HR and ＋ dp/dtmax were increased on the OUA 10 -8 mol/L and 10-7 mol/L groups（P 〈 0.05 or P 〈 0.01,respectively）;The activity of Na＋-K＋-ATPase was significant higher in the OUA 10 -9 mol/L and 10 -8 mol/L groups（P 〈0.05 or P 〈0.01,respectively）.OUA showed dose-dependent inhibition on cardiac function for each group rats with OUA concentration changing from 10 -6 mol/L to 10 -4 mol/L（P 〈 0.01）.The arrhythmia score was also enhanced significantly（P 〈0.01）,and the LDH level in coronary effluent was increased significantly.The activity of Na＋-K＋-ATPase of myocardial tissues showed a dose-dependent reduction（P 〈 0.05 or P 〈 0.01 respectively）.Conclusion：Low concentration of OUA[（10 -9 10 -7 mol/L）]can strengthen the cardiac function of the isolated heart,whereas excessive OUA[（10-610 -4 mol/L）]would inhibit the cardiac function in dose-dependent manner.

关 键 词：哇巴因 离体心脏 大鼠 浓度依赖 

分 类 号：R541.7[医药卫生—心血管疾病]